ASyO2 | Mouse anti-human alpha-synuclein | oligomer-specific
AS13 2717 | clonality: monoclonal | host: mouse | reactivity: human
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|Recommended dilution||1-2 ug/ml (Dot), 2-4 ug/ml (ELISA capture), 10 ug/ml (IHC)|
|Expected | apparent MW||
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Selected references||Brännström et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.|
Dot blot reaction of the binding capacity of ASyO2 to fibrils, monomers and oligomers. Equal amounts of each sample were spotted on a nitrocellulose membrane and then dried. The membrane was blocked with 5% non-fat milk before incubated for 1 h with anti-ASyO2 (25nM) and then with secondary antibody, anti-mouse HRP-conjugated (1:1500). The membrane was washed with PBS containing 0.25% Tween-20 before detection using ECL prime (GE Healthcare).
Tissue sections from the human PD midbrain, substantia nigra, were de-waxed and rehydrated in ethanol and then incubated with ASyO2 at RT for 1h. The immunoreactivity was detected with the anti-mouse Peroxidase Reagent Kit (ImmPRESS, Vector Laboratories, Inc.) and then developed using the ImmPACT AEC Peroxidase Substrate kit (Vector Laboratories, Inc.).
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