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ASyO5 | Mouse anti-human alpha-synuclein | oligomer-specific

540 €

AS13 2718  | clonality: monoclonal  |  host: mouse  |  reactivity: human

 

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AS13 2718

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product information
Background

Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. In analogy to many other amyloid associated disorders, alpha-synuclein may also form oligomeric assemblies. These small and soluble forms have been suggested to exert a stronger tissue damaging effect as compared to the monomeric and fibrillar counterpart. Using a recently developed technique a monoclonal oligomer-specific antibody has been designed.  

Immunogen

synthetic peptide derived from human alpha-synuclein Gly111–Tyr125 

Host Mouse
Clonality Monoclonal
Clone IgG1, (clone number 2.4)
Purity Affinity purified
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage

For short time storage add sodium azide and store at +4°C.
For long time storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), immunohistochemistry (IHC), dot blot (Dot)
Related products

AS13 2719 | anti-ASyM | Mouse anti-human alpha synuclein N-terminal, monoclonal antibodies

AS13 2717B | anti-ASyO2-biotinylated | Mouse anti-human alpha-synuclein | oligomer-specific, monoclonal antibodies
AS13 2718B | anti-ASyO5-biotinylated |  Mouse anti-human alpha-synuclein | oligomer-specific, monoclonal antibodies
AS13 2719B | anti-ASyM-biotinylated | Mouse anti-human alpha synuclein N-terminal, monoclonal antibodies

AS08 358 | anti-SNCA | Alpha-synuclein, rabbit antibodies

Secondary antibodies

Additional information

This antibody is specific to oligomers in ELISA as a capture antibody. For specific details, please check: Brännström et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.

application information
Recommended dilution

2-4 ug/ml (ELISA capture), 10 ug/ml (IHC), 1-2 ug/ml (Dot)

Expected | apparent MW

14 kDa

Confirmed reactivity

human

Predicted reactivity

mouse

Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

to be added when available

Selected references

Brännström et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.


application example

dot blot
dot blot using ASyO5
Dot blot reaction of the binding capacity of ASyO5 to fibrils, monomers and oligomers. Equal amounts of each sample were spotted on a nitrocellulose membrane and then dried. The membrane was blocked with 5% non-fat milk before incubated for 1 h with anti-ASyO5 (25nM) and then with secondary antibody, anti-mouse HRP-conjugated (1:1500). The membrane was washed with PBS containing 0.25% Tween-20 before detection using ECL prime (GE Healthcare).


Immunolocalization

immunolocalization using anti-ASyO5 antibodies

Tissue sections from the human PD midbrain, substantia nigra, were de-waxed and rehydrated in ethanol and then incubated with ASyO5 at RT for 1h. The immunoreactivity was detected with the anti-mouse Peroxidase Reagent Kit (ImmPRESS, Vector Laboratories, Inc.) and then developed using the ImmPACT AEC Peroxidase Substrate kit (Vector Laboratories, Inc.).



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