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Donkey anti-Goat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse, rabbit, rat IgG

203 €

AS10 1069   | clonality: polyclonal  |  host: donkey |  reactivity:goat IgG (H&L)

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AS10 1069

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product information
Background Donkey anti-goat IgG (H&L), HRP conjugated, is a secondary antibody which reacts with all goat IgGs in immunological assays. Antibodies are adsorbed against human,mouse,rabbit,rat IgG and affinity purified using solid phase goat IgG.
Host Donkey
Clonality Polyclonal
Clone
Purity Affinity purified IgG
Format Liquid
Quantity 1 mg (1.0 mg/ml)
Reconstitution
Storage Store undiluted liquid at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products other HRP conjugated secondary antibodies to goat IgG
Additional information

This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse, rabbit, or rat based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

application information
Recommended dilution Needs to be determined by end user for all immunoassay applications.
Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Selected references


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