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FBA | Fructose-bisphosphate aldolase 1, chloroplastic

265 €
AS16 4093 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Festuca arundinacea, Lolium multiflorum

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AS16 4093

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product information
Background FBA (Fructose-bisphosphate aldolase, chloroplastic) is an enzyme involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Immunogen KLH-conjugated peptide chosen from chloroplast fructose-1,6-bisphosphate aldolase (FBA) EC 4.1.2.13 chosen from Brachypodium distachyon FBA protein sequence. UniProt: I1IN47
Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products AS13 2731 | Anti-FBA | Fructose-bisphosphate aldolase class 2, rabbit antibodies

AS08 294 | Anti-ALD | fructose-1,6 bisphosphate aldolase, rabbit antibodies

antibodies involved in carbohydrate metabolism
Additional information
application information
Recommended dilution 1 :4000-1 : 8000 (WB)
Expected | apparent MW 42 | 38 kDa
Confirmed reactivity Festuca arundinacea, Lolium multiflorum
Predicted reactivity Aegilops tauschii, Arundo donax, Avena sativa, Brachypodium distachyon, Cajanus cajan, Catharanthus roseus, Carica papaya , Citrus sinensis, Cucumis sativus, Eucalyptus grandis, Genlisea aurea, Glycine soja , Gossypium raimondii, Hordeum vulgare var. distichum, Jatropha curcas, Leersia perrieri, Manihot esculenta, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Populus trichocarpa, Ricinus communis, Setaria italica, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Trifolium pRatense, Triticum aestivum, Zea mays, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information Standard extraction protocol for leaf tissue can be applied. The antibody is directed on the unique peptide present in a chloroplast form of aldolase; it does not react with a cytosolic form in the Lolium-Festuca species.
Selected references Perlikowski et al. (2016). Water deficit affects primary metabolism differently in two Lolium multiflorum/Festuca arundinacea introgression forms with a distinct capacity for photosynthesis and membrane regeneration. Frontiers in Plant Science 7:1063. doi: 10.3389/fpls.2016.01063

Application example

western blot using anti-FBA, cytoplasmic aldolase antibodies


50-100 µg of total protein from Oryza sativa cv. 9311 seed at the maturing stage, extracted with protein extraction reagent were separated on 15% SDS-PAGE and blotted 1h to PVDF using wet transfer. Blots were blocked with 1×TBST+5% Nonfat dry milk for 1h at 37°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 in 1×TBST+5% Nonfat dry milk for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:15 000 in 1x TBST + 5% Nonfat dry milk for 2h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10-20 seconds.

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