GFP | Green Fluorescence Protein (protein A purified)
AS15 3000 | clonality: polyclonal | host: rabbit | reactivity: native and recombinant GFP
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|Recommended dilution||1 : 5000-1 : 25 000 (ELISA), 1 : 2000-1 : 10 000 (WB)|
|Expected | apparent MW|
|Confirmed reactivity||Native GFP, Recombinant GFP (E.coli), all variants of GFP and EGFP|
|Not reactive in|
Minimal cross-reactivity with E.coli proteins
to be added when available, antibody released in September 2015
EGFP-tagged nuclear lamins (in green), detected in the same cell by anti-GFP | Green Fluorescence Protein antibodies (in red; dilution 1:500).
Mouse fibroblasts were transfected with an EGFP-Lamin A hybrid construct and indirectly immunostained using anti-GFP | Green Fluorescence Protein as the primary antibody. Fixation and permeabilization was performed with 4% buffered formaldehyde for 10 min. at room temperature and treatment with 0.1% Triton X-100 for 10 min., respectively.
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