GI | Gigantea

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AS12 1864 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


35 st
Item No:
AS12 1864A

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product information
Background GI (GIGANTEA) is involved in several developmental processes including regulation of circadian rhythm and photoperiodic flowering, phytochrome B signaling, circadian clock, carbohydrate metabolism and cold stress response.
Immunogen KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana GI, UniProt:Q9SQI2,TAIR: AT1G22770
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution please add 50 ĩl of dest. water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.

Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1: 1000 with ECL (WB)
Expected | apparent MW

127.9 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Brassica campestris, Chrysanthemum morifolium, Dimocarpus longan, Festuca pratensis, Gentiana triflora, Glycine soja, Hordeum vulgare, Liriodendron tulipifera, Lolium perenne, Lotus japonicus, Medicago truncatula, Plantago major, Populus balsamifera, Prunus dulcius, Ricinus communis, Secale cereale, Theobroma cacao, Triticum aestivum
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references

to be added when available, antibody released in January 2016. 

application example

western blot using anti-GI antibodies

50 µg of total protein from Arabidopsis thaliana extracted with TRIZOL protocol and finally dissolved in buffer E (Martínez-García et al., 1999, Plant J 20:251-7), was denatured with SDS at 95 C for 5 min, were separated on 12% (w/v) acrylamide/bis-acrylamide SDS-PAGE and blotted 10 mins to nitrocellulose using semi-dry tank transfer. Blots were blocked with 5% (w/v) skimmed milk in TBSt (Tris-Buffer Saline + 0.1% (v/v) tween-20) for 2h at room temperature (RT) with agitation. TBSt Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 4 times for 15 min in TBSt at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in TBSt for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL (Life Science). Exposure time was continuous for 10 mins in a CCD camera. The image was taken after 5 min exposure.

Courtesy of Dr. Federico Valverde Albacete, CSIC, Spain

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