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Goat anti-Chicken IgY (H&L), DyLightŪ 350 conjugated

181 €

AS11 1820| clonality: polyclonal  |  host: goat  | reactivity: chicken IgY (H&L)

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AS11 1820

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product information
Background

Goat anti-chicken IgY, DyLight® 350 Conjugate is a secondary antibody conjugated to DyLight® 350, which binds to hen immunoglobulins in immunological assays.

DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Immunogen

purified chicken IgY, whole molecule

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications immunohistochemistry (IHC), immunocytochemistry (ICC)
Related products

other anti-chicken Dylight conjugated secondary antibodies

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

DyLight® 350  (Ex = 353 nm; Em = 432 nm).

application information
Recommended dilution

1:20 – 1:2 000 (IHC), 1:50 – 1:5 000 (ICC)

Expected | apparent MW
Confirmed reactivity

chicken IgY heavy and light chains (H&L)

Predicted reactivity

chicken IgY heavy and light chains (H&L)

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgG and light chains on all chicken immunoglobulins.

No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.

Selected references

to be added when available



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