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Goat anti-Human IgG Fc, F(ab)'2 fragment, HRP conjugated, min. cross-reactivity bovine/mouse/rabbit serum

203 €

AS10 817 | clonality: polyclonal  |  host: goat  | reactivity: human IgG Fc (two heavy chains with constant domains)

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AS10 817

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product information
Background

Goat anti-human IgG Fc (two heavy chains with constant domains) F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.

Immunogen

Purified human IgG

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 0.5 mg
Reconstitution For reconstitution please add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
Related products

antibodies to human immunoglobulins

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

application information
Recommended dilution

optimal working dilution should be determined by the investigator

Expected | apparent MW
Confirmed reactivity human IgG Fc (two heavy chains with constant domains)
Predicted reactivity

human IgG Fc (two heavy chains with constant domains)

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.

No reactivity is observed with the light chains on human immunglobulins or non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins or IgG F(ab)' fragment based on immunoelectrophoresis.

The antibody ahs been absorbed against solid phase normal bovine, mouse and rabbit serum in addition to mouse and rabbit IgG.

Selected references

to be added when available



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