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Goat anti-Human IgG (H&L), F(ab)'2 fragment, ALP conjugated

181 €

AS10 774 | clonality: polyclonal  |  host: goat  | reactivity: human IgG (H&L)

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AS10 774

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product information
Background

Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L) in immunological assays.

Immunogen

Purified human IgG

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified goat IgG, F(ab)'2 fragment
Format Liquid
Quantity 0.5 mg
Reconstitution
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC)
Related products

other secondary antibodies to human immunoglobulins

Additional information

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.

Purity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.

application information
Recommended dilution

1: 500 - 2 000 (ELISA) and  (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)

Expected | apparent MW
Confirmed reactivity

human IgG (H&L)

Predicted reactivity

human IgG (H&L)

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

This antibody reacts with the alpha chains on human IgG based on immunoelectrophoresis.

No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.

Selected references

to be added when available



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