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GUS | Beta-glucuronidase

265 €
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AS16 3689 | Clonality: Polyclonal | Host: Rabbit | Reactivity: GUS from Escherichia coli

PRODUCT INFORMATION IN PDF

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AS16 3689

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product information
Background GUS (Beta-glucuronidase) is an enzyme which when incubated with colorless or non-fluorescent substrates, can catalyze reaction of their transformation into coloured or fluorescent products.
Immunogen

KLH-conjugated synthetic peptide derived from Escherichia coli GUS protein

Host Rabbit
Clonality Polyclonal
Clone
Purity

Serum

Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications

Western Blot (WB)

Related products Antibodies to other reporter proteins

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 10 000 (WB)

Expected | apparent MW

depends upon a MW of a protein which is expressed with GUS

Confirmed reactivity GUS
Predicted reactivity
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information
Selected references

To be added when available, antibody released in July 2017.


Application example

western blot using anti-GUS antibodies 

100 µg of total protein from Arabidopsis thaliana wild-type (1) and transgenic plants expressing GUS under HRT promoter (2) were extracted with write buffer containing  50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10 mM MgCl2, 5 mM EDTA, 10% glycerol, 0.1% NP-40, 0.1% protease inhibitor cocktail . The proteins were denatured by boiling for 5 minwere separated on 10% SDS-PAGE  and blotted 30 min to PVDF using tank transfer. Blots were blocked with  TBST containing 5% non-fat dry milk   for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 2h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 15 min each in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed with  Clarity MAX (Bio-RAD). Exposure time was 10 seconds.

Courtesy of Dr. Pradeep Kachroo, University of Kentucky, USA

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com