AtpA | alpha subunit of ATP synthase

320 €

AS08 304 | clonality: polyclonal | host: rabbit | reactivity: A.thaliana, Z.mays

product information

background

ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.

immunogen

recombinant maize chloroplast AtpA P05022

antibody format

rabbit;

polyclonal;

serum;

lyophilized

quantity

100 µl

- for reconstitution add 100 µl of sterile water

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

Western blot (WB)

related products

AS03 030 | anti-ATP synthase subunit beta hen antibody

AS08 312 | anti-ATP synthase subunit gamma antibody

AS05 071 | anti-ATP synthase subunit c antibody

AS08 370 | anti-ATP synthase whole enzyme

additional information

sequence of protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757

application information
recommended dilution		1: 10 000 with standard ECL (WB)
expected \| apparent MW		55 kDa
confirmed reactivity		Arabidopsis thaliana, Zea mays
predicted reactivity		established to react with dicot and monocot chloroplast AtpA; may cross-react with mitochondrial AtpA; algae, cyanobacteria
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		to be added when available
selected references		to be added when available

application example

Western blot

Lane 1: Arabidopsis thaliana total leaf protein extract;

Lane 2: Zea mays total leaf protein extract

5 µg of total protein were loaded per each lane.

Experimental conditions:

Proteins were separated in a 12% or 5-15% gradient gel following by a transfer to nitrocellulose membrane. Membrane was blocked with TBST + 4% non-fat dried milk, 20 min following by three washes in TBST. Incubation time with primary and secondary antibodies was 1 hr primary, 30 min for secondary antibodies. Manufacturer of secondary antibodies: Bio-Rad

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