Plant/Algal cell antibodies / Bioenergetics

product information

ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.

Alternative names: ATPase subunit II, ATP synthase F(0) sector subunit b'

KLH-conjugated mix of synthetic peptide derived from Arabidopsis thaliana AtpG Q0WMW8 and Chlamydomonas reinhardtii ATP synthase subunit b' A8J785

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Western blot (WB)

AS08 370 | anti-ATP synthase, whole enzyme

AS08 304 | anti-ATP synthase subunit alpha antibody

AS03 030 | anti-ATP synthase subunit beta hen antibody

AS05 071 | anti-ATP synthase subunit c antibody

AS08 312 | anti-AtpC | gamma subunit of ATP synthase

AS07 260 | H+ATPase | plasma membrane H+ATPase

to be added when available

application information

1: 10 000 (WB)

22.4 kDa (Chlamydomonas reinhardtii) 15.9 kDa (Arabidopsis thaliana)

Arabidopsis thaliana, Chlamydomonas reinhardtii

dicots including: Sorghum bicolor,  monocots including: Oryza sativa, Zea mays, algae Volvox carteri

no confirmed exceptions from predicted reactivity known in the moment

Antibody reactivity for Arabidopsis thaliana has been confirmed on membrane fraction.

to be added when available. Antibodies released in November 2009.

application example

10 ug of chlorophyll/well of Chlamydomonas reinhardtii total cell extract (1), Chlamydomonas reinhardtii subunit II deletion mutant thylakoid membrane fraction (2), Arabidospsis thaliana thylakoid membrane fraction (3), were separated on 12-18% acrylamide-8M urea gel and blotted to nitrocellulose membrane. Filters were blocked 1 h with 5% dry milk in 1 x PBS and probed with anti-ATP synthase subunit gamma antibody (AS09 457, 1: 10 000, 1h) and secondary HRP-conjugated anti-rabbit antibody (1: 10 000, 1 h) in 1 x PBS containing 5% dry milk. All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare), exposure time was 1 min.

Arabidopsis membrane preparation has been done according to Lezhneva et al. (2008) A novel pathway of cytochrome c biogenesis is involved in the assembly of the cytochrome b6f complex in arabidopsis chloroplasts. J Biol. Chem., 283:24608-24616 and Chlamydomonas membranes were prepared according to Chua & Bennoun (1975) Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. PNAS 72:2175-2179

Courtesy Dr. Yves Choquet