FT/TSF | flowering locus T and twin sister of FT

320 €

AS06 198 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

product information

background

In angioperms the 20 kDa protein FT (coded by the gene flowering locus T) promotes flowering under long-day conditions. It has been shown to contribute to flowering initiation upon its expression in leafs followed by transport to apical meristems.

immunogen

KLH-conjugated synthetic peptide derived from A.thaliana FT protein sequence (Q9SXZ2, At1g65480); please note that this antibody will cross-react with the highly homologous TSF (twin sister of FT) protein

antibody format

rabbit

polyclonal

Affinity puurified serum

lyophilized

quantity

200 µg

for reconstitution add 100 µl of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products

antibodies to proteins involved in photomorphogenesis

additional information

to be added when available

application information
recommended dilution		1 : 1000, detected with standard ECL (WB)
expected \| apparent MW		20 \| 20 kDa for Arabidopsis thaliana
confirmed reactivity		Arabidopsis thaliana
predicted reactivity		Arabidopsis thaliana
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		note that detection for this product is limited by target threshold level
selected references		to be added when available

application example

35 µg of total leaf protein extracted with PEB (AS08 300) from wt Arabidopsis thaliana (1) and Arabidopsis thaliana transformed with 35S::FT (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 80 min (30V) to PVDF. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-FT/TFT (AS06 198, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (300s, high sensitivity). Please note that this antibody will not detect FT at 35 µg protein loading in the wt leaf material tested.

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