Plant/Algal cell antibodies / Environmental stress / Heat shock

product information

HSP90C is a nuclear-encoded, chloroplast-targeted chaperone of the HSP90 family. It forms dimers that interact with HSP70B. HSP90C displays a weak ATPase activity that is inhibited by radicicol.

C-terminal 238 amino acids of HSP90C, Q66T67, expressed with N- and C-terminal hexahistidine tags in E. coli, purified with Ni-NTA

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB) immunoprecipitation (IP)

AS08 346 | HSP90-1 | heat shock protein 90-1 with reactivity to higher plants

AS11 1629 | HSP90-2 | heat shock protein 90-2 with reactivity to higher plants

collection of antibodies to other heat shock proteins

collection of antibodies to Chlamydomonas reinhardtii

to be added when available

application information

1 : 3000 with standard ECL (WB)

89 kDa

Chlamydomonas reinhardtii

Ostreococcus sp.

no confirmed exceptions from predicted reactivity known in the moment

HSP90C protein is easily degraded and degradation products are detected by this antibody.

Willmund &  Schroda (2005). HSP90C is a bona-fide Hsp90 that interacts with plastidic HSP70B in Chlamydomonas reinhardtii. Plant Phys. 138, 2310–2322.