Plant/Algal cell antibodies / Environmental stress / Heat shock

product information

CDJ1 is one of three zinc-finger containing J domain proteins of Chlamydomonas reinhardtii. This protein is found in chloroplast. In spite that CDJ1 gene is heat shock inducible it does not reflect in increased CDJ1 protein levels. CDJ1 forms complexes with plastidic HSP90C,HSP70B and CGE1 and is a new cohort protein of the chloroplast multi-chaperone complex.DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation.

fusion protein encoding region for the Chlamydomonas rerinhardtii N-terminal part of CDJ1 Q66YD3, overexpressed in E.coli

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB) immunoprecipitation (IP)

collection of antibodies to other heat shock proteins

collection of antibodies to Chlamydomonas reinhardtii

collection of antibodies to chloroplast proteins

to be added when available

application information

1. 3000 with standard ECL (WB)

40 | 40 kDa

Chlamydomonas reinhardtii

Ostreococcus sp.

higher plants

western blot has been done on 10-50 µg load per well; immunoprecipitation has been done on 5-50 µl

Willmund et al (2008). The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C. Plant Physiol. 148: 2070-2082.