Plant/Algal cell antibodies / Membrane transport system / Endomembrane system

product information

Sec21p is a constituent of the COPI vesicle coatomer.

GST fusion of a part of recombinant Sec21 of Arabidopsis thaliana Q0WW26

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), immunofluorescence (IF)

AS10 683 | anti-HDEL | endoplasmic reticulum retention signa, 100 µg

AS10 683-25 | anti-HDEL | endoplasmic reticulum retention signal, 25 µg

AS08 325 | anti-Arf1 | ADP-ribosylation factor 1, Golgi marker, rabbit antibody

to be added when available

application information

1: 1000 with standard ECL (WB), 1: 1000 (IF)

98 kDa

Arabidopsis thaliana, Zea mays

dicots including Vitis vinifera, monocots inclusing Oryza sativa, Zea mays, deciduous broadleaf tree Populus trichocarpa

Nicotiana tabacum, Microsporidia sp.

this antibody can be used as a Golgi marker in immunolocalization and as a marker of COP1 in Western blot

Movafeghi et al. (1999) Arabidopsis Sec21p and Sec23p homologs: Probable coat proteins of plant COP-coated vesicles. Plant Physiol. 119, 1437–1445.

application example

50 µg of total protein from (1) Nicotiana tabacum protoplast total protein, (2) Arabidopsis thaliana protoplast soluble protein, (3) Arabidopsis thaliana protoplast total protein were separated on  10 % SDS-PAGE and blotted 2h to nitrocellulose (Semi-dry, 200mA). Filters were blocked over night with 5% low-fat milk powder in TBS and probed with anti-Sec21p antibodies (AS08 327, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP) in TBS-Tween. Signal was detected with standard ECL and exposure time for this image was 1 minute.

Protoplasts were extracted in 50mM Tris, 10 mM EDTA and Triton X100, 0.02%.