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product information
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| background |
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eEF1B-alpha2 protein belongs to a family of elongation factors, proteins which are involved in translational elongation. Alternative names: Elongation factor 1-beta 2, Elongation factor 1-beta' 2, EF-1-beta' 2 |
| immunogen |
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recombinant eEF1B-alpha2 protein from Arabidopsis thaliana with no affinity tag, Q9SCX3 |
| antibody format |
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rabbit |
polyclonal |
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serum |
lyophilized |
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| quantity |
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200 µl |
for reconstitution add 200 µl of sterile water |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS11 1633 | EF1A | elongation factor 1-alpha / EF-1-alpha
AS10 678 | eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 2 AS10 677 | eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2 AS07 265 | eEF1b | elongation factor eEF1b-beta protein AS10 676 | eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2collection of antibodies to DNA/RNA metabolism |
| additional information |
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Antibody shows a very weak cross-reaction to elongation factor 1B-alpha 1. |
application information
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| recommended dilution |
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1: 2000 with standard ECL (WB) |
| expected | apparent MW |
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24 | 34 kDa |
| confirmed reactivity |
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Arabidopsis thaliana |
| predicted reactivity |
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dicots including: Glycine max, Solanum tuberosum, Vitis vinifera, monocots including: Oryza sativa, Zea mays |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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to be added when available, antibody released in 2010 |
application example
 10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (1) or heat treated at 38ºC for 2 hours prior to protein extraction (2). As positive control 10 ng of recombinant elongation factor proteins alpha 1 (3) and alpha 2 (4) were separated side by side with the plant samples on 11% SDS-PAGE and blotted to nitrocellulose (Bio-rad). Blots were blocked following transfer with 5% low fat milk in low salt buffer for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 2h at room temperature with agitation in the blocking solution. The primary antibody solution was removed and the blot was rinsed briefly twice, then washed 4 times for 15 min each at room temperature with agitation using low salt buffer. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxides conjugated, from Amersham diluted to 1:2500 for 1h at room temperature with agitation then washed as above and treated with ECL detection reagent according to the manufacturers instructions. Exposure time was 5 seconds. The primary antibody could be reused if it is kept at 4ºC for 2 weeks and if frozen at -20ºC for long time. The pre-immune did not cross react with any plant protein non-specifically. Low salt buffer components are 10 mM Tris (pH 7.6), 68 mM NaCl and 0.05 % Triton X-100.
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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