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Lhcb2 | LHCII type II chlorophyll a/b-binding protein

305 €

AS01 003  |  clonality: polyclonal  |  host: rabbit  |  reactivity: photosynthetic eukaryotes including A. thaliana, A. hypogaea, C. quitensis Kunt Bartl, H. vulgare, L. esculentum (Solanum lycopersicon), M. crystallinum, N. tabacum, O. sativa, P. sativum, P. vulgaris, S. oleracea, Z. mays, P. patens and Ch. reinhardtii

PRODUCT INFORMATION IN PDF

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AS01 003

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product information
background  

The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb2 is often coded by several nuclear genes and is found together with Lhcb1 within the mobile LHCII trimers involved in state1-state2 transition.
A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII:  Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467–9476.

immunogen  

BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhcb2 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana Lhcb2.1 UniProt: Q9SHR7, TAIR: AT2G05100, Lhcb2.2 UniProt: Q9S7J7, TAIR:AT2G05070, Lhcb2.3 UniProt:Q9XF87, TAIR:AT3G27690
 

antibody format  

rabbit

polyclonal,

total IgG in PBS pH 7.4,

lyophilized

quantity  

200 µl

- for reconstitution add 200 µl of sterile water

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB), immunoprecipitation (IP)

related products   AS13 2705 | Lhcb2 | LHCII type II chlorophyll a/b-binding protein, phosphorylated, rabbit antibody

AS01 002  Lhcb3 | LHCII type III chlorophyll a/b-binding protein

AS01 004  Lhcb1 | LHCII type II chlorophyll a/b-binding protein

All anti-LHC antibodies

Antibodies to other proteins involved in photosynthesis

additional information  

Antibody format is a total IgG fraction, which means that it is a pool of polyclonal antibodies obtained by purification of serum on Protein G, not on a specific antigen column.

application information
recommended dilution  

1 : 5000 with standard ECL (WB), 5 µl of antibody solution (IP)

expected | apparent MW  

25 | 25 kDa for Arabidopsis thaliana

confirmed reactivity  

Arabidopsis thaliana, Arachis hypogaea, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Spinacia oleracea, Zea mays, Physcomitrella patens, Chlamydomonas reinhardtii

predicted reactivity  

angiosperms (monocots and dicots), gymnosperms, mosses, green algae

not reactive in  

no confirmed exceptions from predicted reactivity known at the moment

additional information  

Immunoprecipitation has been done using Immunoprecipitation kit from Roche, Cat.No. 11 719 386 001.

selected references  

Wientjes et al (2013). LHCII is an antenna of both photosystems after long-term acclimation. BBA, Jan 6.

Albus et al. (2012). LCAA, a novel factor required for Mg protoporphyrin monomethylester cyclase accumulation and feedback-control of aminolevulinic acid biosynthesis in tobacco. Plant Physiol. Oct 19.

Rudowska et al. (2012). Chloroplast biogenesis - correlation between structure and function. BBA, available on line, March 2012.

Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).



||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at  support@agrisera.com

Do you have a matching secondary antibody?

AS09 602 | Goat anti-rabbit IgG (H&L), HRP conjugated

AS09 607 | Goat anti-rabbit IgG (H&L), ALP conjugated 

AS09 603 | Goat anti-chicken IgY (H&L), HRP conjugated

AS09 606 | Goat anti-chicken IgY (H&L), ALP conjugated

For a complete list of Agrisera secondary antibodies


Protein extaction buffer:

AS08 300 | Plant and Algal Protein Extraction Buffer (PEB)
An extraction buffer for quantitative isolation of total soluble/membrane protein from various tissues, optimized for subsequent western blot detection.