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Lhcb2 | LHCII type II chlorophyll a/b-binding protein

323 €

AS01 003  |  clonality: polyclonal  |  host: rabbit  |  reactivity: photosynthetic eukaryotes including A. thaliana, A. hypogaea, C. quitensis Kunt Bartl, H. vulgare, L. esculentum (Solanum lycopersicon), M. crystallinum, N. tabacum, O. sativa, P. sativum, P. vulgaris, S. oleracea, Z. mays, P. patens and Ch. reinhardtii

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AS01 003

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Background

The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb2 is often coded by several nuclear genes and is found together with Lhcb1 within the mobile LHCII trimers involved in state1-state2 transition.
A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII:  Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467–9476.

Immunogen

BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhcb2 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana Lhcb2.1 UniProt: Q9SHR7, TAIR: AT2G05100, Lhcb2.2 UniProt: Q9S7J7, TAIR:AT2G05070, Lhcb2.3 UniProt:Q9XF87, TAIR:AT3G27690
 

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications

western blot (WB), immunoprecipitation (IP)

Related products

AS01 002 | Lhcb3 | LHCII type III chlorophyll a/b-binding protein

AS01 004 | Lhcb1 | LHCII type I chlorophyll a/b-binding protein

AS13 2705 | Lhcb2-P | LHCII type II chlorophyll a/b-binding protein, phosphorylated

Additional information

Antibody format is a total IgG fraction, which means that it is a pool of polyclonal antibodies obtained by purification of serum on Protein G, not on a specific antigen column.

application information
Recommended dilution

1 : 5000 with standard ECL (WB), 5 µl of antibody solution (IP)

Expected | apparent MW

28.6 | 25 kDa for Arabidopsis thaliana

Confirmed reactivity

Arabidopsis thaliana, Arachis hypogaea, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Miscanthus x giganteus, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Spinacia oleracea, Zea mays, Physcomitrella patens, Chlamydomonas reinhardtii

Predicted reactivity

angiosperms (monocots and dicots), gymnosperms, mosses, green algae

Not reactive in

no confirmed exceptions from predicted reactivity known at the moment

Additional information

Immunoprecipitation has been done using Immunoprecipitation kit from Roche, Cat.No. 11 719 386 001.

Protein is processed into mature form (Jansson 1999).

Selected references Kowalewska et al. (2016). Three-dimensional visualization of the internal plastid membrane network during runner bean chloroplast biogenesis. Dynamic model of the tubular-lamellar transformation. The Plant Cell March 21, 2016 tpc.01053.2015.
Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113.
Liu and Last (2015). A land plant-specific thylakoid membrane protein contributes to photosystem II maintenance in Arabidopsis thaliana. Plant J. 2015 Jun;82(5):731-43. doi: 10.1111/tpj.12845. Epub 2015 Apr 29.
Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675.
Spence et al. (2014). Transcriptional responses indicate maintenance of photosynthetic proteins as key to the exceptional chilling tolerance of C4 photosynthesis in Miscanthus × giganteus. J Exp Bot. 2014 Jul;65(13):3737-47. doi: 10.1093/jxb/eru209. Epub 2014 Jun 22.
Saito et al. (2014). Fe deficiency induces phosphorylation and translocation of Lhcb1 in barley thylakoid membranes. FEBS Lett. 2014 May 8. pii: S0014-5793(14)00317-2. doi: 10.1016/j.febslet.2014.04.031.
Wientjes et al (2013). LHCII is an antenna of both photosystems after long-term acclimation. BBA, Jan 6.


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