Western blot Lane 1: Arabidopsis thaliana total leaf protein extract; Lane 2: Zea mays total leaf protein extract 5 µg of total protein were loaded per each lane. Experimental conditions: Proteins were separated in a 12% or 5-15% gradient gel following by a transfer to nitrocellulose membrane. Membrane was blocked with TBST + 4% non-fat dried milk, 20 min following by three washes in TBST. Incubation time with primary and secondary antibodies was 1 hr primary, 30 min for secondary antibodies. Manufacturer of secondary antibodies: Bio-Rad |