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product information
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| background |
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Cytochrome c6 is known as a redox carrier of the thylakoid lumen of cyanobacteria and some eukaryotic algae that can substitute for plastocyanin in electron transfer.
Cytochrome c6 is situated between the two membrane-bound complexes cytochrome b6f and photosystem I (PSI) in oxygenic photosynthesis..
Cytochrome c6 (cyt c6) in Chlamydomonas reinhardtii is synthesized only under conditions of copper deficiency when plastocyanin cannot be synthesized. |
| immunogen |
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GST fusion of recombinant Chlamydomonas reinhardtii cytochrome c6 A8J9G6 |
| antibody format |
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rabbit |
polyclonal |
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serum |
lyophilized |
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| quantity |
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200 µl |
for reconstitution add 200 µl of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS06 122 | anti-CRD1 | cyanobacterial homolog of plant CHL27 cyclase, marker of thylakoid and envelope membranes AS05 084 | anti-PsbA rabbit antibody, marker of thylakoid membrane Collection of antibodies to Chlamydomonas proteins |
| additional information |
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cellular [compartment marker] of thylakoid lumen for Chlamydomonas reinhardtii |
application information
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| recommended dilution |
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1 : 1 000 with alkaline phosphatase (WB) |
| expected | apparent MW |
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15 kDa |
| confirmed reactivity |
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Chlamydomonas reinhardtii |
| predicted reactivity |
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Chlorella vulgaris, Pediastrum duplex, Cladophora glomerata |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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Note: antibody will work better with nitrocellulose than with PVDF membrane. Recommended load per well min. 6 µg. Primary antibody incubation needs to be done over night. For detection image please refer to the reference below |
| selected references |
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Merchant et al. (1998) Copper-Responsive Gene Expression in Photosynthetic Microorganisms. Methods in Enzymology 297:263-279. |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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