Plant/Algal cell antibodies / Photosynthesis / Chlorophyll

product information

PBG is an enzyme involved in tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.

overexpressed Thermosynechococcus elongatus HemC Q8DIE4

store lyophilized/reconstituted at -20�°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS07 226 | anti-HemF

AS07 228 | anti-HemH | protoporphyrin ferrochelatase

to be added when available

application information

1: 1000 - 1: 5000 (WB)

34.7 kDa

Thermosynechococcus elongatus

cyanobacteria

Synechococcus sp. PCC 6803

to be added when available

to be added when available

application example

0.5 ug chl from Synechocystissp. PCC 6803 membrane fraction (1) and soluble fraction (2) and Thermosynechococcus elongatus (3); Synechocystis was prepared as a soluble and membrane fractions. Separated on 12% SDS-PAGE and blotted overnight at 4C to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS. Probed with anti-HemC antibody in TTBS (AS08 357, 1:2000, 4h) and secondary anti-rabbit in TTBS (1:10000,1h). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000.

Courtesy Dr. Roman Sobotka Laboratory of Photosynthesis, Trebon