HemC | porphobilinogen-Deaminase (PBG)

320 €

AS08 375 | clonality:polyclonal | host:rabbit | reactivity:Thermosynechoccus elongatus

product information

background

PBG is an enzyme involved in tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.

immunogen

overexpressed Thermosynechococcus elongatus HemC Q8DIE4

antibody format

rabbit

polyclonal

serum

lyophilized

quantity

200 µl

for reconstitution add 200 µl of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products

AS07 226 | anti-HemF

AS07 228 | anti-HemH | protoporphyrin ferrochelatase

additional information

to be added when available

application information
recommended dilution		1: 1000 - 1: 5000 (WB)
expected \| apparent MW		34.7 kDa
confirmed reactivity		Thermosynechococcus elongatus
predicted reactivity		cyanobacteria
not reactive in		Synechococcus sp. PCC 6803
additional information		to be added when available
selected references		to be added when available

application example

0.5 ug chl from Synechocystissp. PCC 6803 membrane fraction (1) and soluble fraction (2) and Thermosynechococcus elongatus (3); Synechocystis was prepared as a soluble and membrane fractions. Separated on 12% SDS-PAGE and blotted overnight at 4C to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS. Probed with anti-HemC antibody in TTBS (AS08 357, 1:2000, 4h) and secondary anti-rabbit in TTBS (1:10000,1h). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000.

Courtesy Dr. Roman Sobotka Laboratory of Photosynthesis, Trebon

wesern blot detection using anti-PBG (HemC) antibodies

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