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product information
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| background |
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GPX belongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX. |
| immunogen |
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thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala of AtGPX7 accession At4g31870 has been used for antibody production |
| antibody format |
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rabbit |
polyclonal |
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serum, |
lyophilized |
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| quantity |
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100 µl, |
for reconstitution add 100 µl, of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) ELISA (ELISA) |
| related products |
|
AS06 181 | anti-glutatione reductase rabbit antibody |
| additional information |
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not available at the moment |
application information
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| recommended dilution |
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1: 5000 with alkaline phosphatase system (AP) (WB), 1:1, 1:2, 1:4, 1:8, 1: 16 (ELISA) |
| expected | apparent MW |
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25 | 20 kDa |
| confirmed reactivity |
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Arabidopsis thaliana |
| predicted reactivity |
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n.a. |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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This antibody is recognizing Arabidopsis thaliana GPX (two chloroplastic isoforms). Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended. |
| selected references |
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Juszczak et al. (2012). Natural genetic variation in the expression regulation of the chloroplast antioxidant system among Arabidopsis thaliana accessions. Physiol. Plant. |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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