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PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII

325 €

AS06 142-16  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana, H. vulgare, Z. mays

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Item No:
AS06 142-16

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product information
background  

PSII reaction centre components are  generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site          of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC. Synonymes: PSBQ, PSBQA

immunogen  

KLH-conjugated synthetic peptide derived from available PsbQ protein sequences including Arabidopsis thaliana At4g21280

antibody format  

rabbit

polyclonal

serum

lyophilized

quantity  

200 µl

for reconstitution add 200 µl of sterile water.

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB)

related products  

AS05 092 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII

AS06 142-33 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII

AS06 167 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII

AS08 305 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII

additional information  

to be added when available

application information
recommended dilution  

1: 1000 with standard ECL (WB)

expected | apparent MW  

23.8 | 16 kDa

confirmed reactivity  

Arabidopsis thaliana, Hordeum vulgare, Pisum sativum, Zea mays

predicted reactivity  

dicots including: Spinacia oleracea, monocots including: Oryza sativa, Triticum aestivum,  trees: Picea sitchensis, Populus balsamifera

not reactive in  

no confirmed exceptions from predicted reactivity known in the moment

additional information  

to be added when available

selected references  

Grassl et al. (2012). Early events in plastid protein degradation in stay-green Arabidopsis
reveal differential regulation beyond the retention of LHCII and chlorophyll. J. Proteome Res. October 2.


application example
western blot using anti-PsbQ antibodies
5 µg of total protein from Arabidopsis thaliana (1), Hordeum vulgare (2), Zea mays (3)   extracted with Agrisera PEB buffer (AS08 300)  were separated on  4-12 % NuPAGE Bis-Tris gel (Invitrogen) and blotted 1h to PVDF. Blots were blocked with ECL Advance blocking reagent   for 1.5 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Agrisera, AS09 602) diluted to 1:25 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 44 seconds.

||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com

Do you have a matching secondary antibody?

AS09 602 | Goat anti-rabbit IgG (H&L), HRP conjugated

AS09 607 | Goat anti-rabbit IgG (H&L), ALP conjugated 

AS10 1489 | Rabbit anti-Chicken IgY (H&L), HRP conjugated 

AS09 606 | Goat anti-chicken IgY (H&L), ALP conjugated

For a complete list of Agrisera secondary antibodies


Detection reagents:

AS14 ECL-100 | AgriseraECL Bright 
AgriseraECL Bright for Western Blot detection is a high quality substrate for detection of horseradish peroxidase enzyme activity at a femtogram level. Its a ready to use 2 component system with low background and superior signal to nouse ratios.

AS14 TMB-HRP | AgriseraTMB HRP Peroxidase Microwell Substrate (100 ml) 
TMB based, one component, especially formulated with extreme sensitivity, HRP substrate for microwell application.