PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII
AS06 142-16 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, H. vulgare, P. abies, Z. mays
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1: 1000 with standard ECL (WB)
|expected | apparent MW||
23.8 | 16 kDa
Arabidopsis thaliana, Hordeum vulgare, Picea abies, Pisum sativum, Zea mays
dicots including: Spinacia oleracea, monocots including: Oryza sativa, Triticum aestivum, trees: Picea sitchensis, Populus balsamifera
|not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
to be added when available
|selected references||Pavlovič et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.
Albanese et al. (2016). Isolation of novel PSII-LHCII megacomplexes from pea plants characterized by a combination of proteomics and electron microscopy. Photosynth Res. 2016 Jan 9.
Grassl et al. (2012). Early events in plastid protein degradation in stay-green Arabidopsis reveal differential regulation beyond the retention of LHCII and chlorophyll. J. Proteome Res. October 2.
5 µg of total protein from Arabidopsis thaliana (1), Hordeum vulgare (2), Zea mays (3) extracted with Agrisera PEB buffer (AS08 300) were separated on 4-12 % NuPAGE Bis-Tris gel (Invitrogen) and blotted 1h to PVDF. Blots were blocked with ECL Advance blocking reagent for 1.5 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Agrisera, AS09 602) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 44 seconds.
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