Plant/Algal cell antibodies / Physcomitrella patens

product information

HSP70B is a nuclear-encoded, chloroplast-targeted chaperone of the HSP70 family. It is the major HSP70 in the stroma of Chlamydomonas reinhardtii chloroplasts. It interacts with HSP90C, CGE1, CDJ2, and VIPP1.

mature HSP70B protein expressed with N- and C-terminal hexahistidine tags in E. coli, purified with Ni-NTA

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB) immunoprecipitation (IP)

collection of antibodies to other heat shock proteins

collection of antibodies to Chlamydomonas reinhardtii

to be added when available

application information

1:10 000 with standard ECL (WB)

71.9 kDa

Chlamydomonas reinhardtii, Desmodesmus subspicatus, Physcomitrella patens

Dunaliella salina, cyanobacteria

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

Diaz-Troya et al. (2011). Inhibition of protein synthesis by TOR inactivation revealed a conserved regulatory mechanism of the BiP chaperone in Chlamydomonas. Plant Physiol, in press.

Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source) Tukaj and Tukaj (2010). Distinct chemical contaminants induce the synthesis of Hsp70 proteins in green microalgae Desmodesmus subspicatus: Heat pretreatment increases cadmium resistance. Journal of Thermal Biology 35 (5):239-244.