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product information
|
| background |
|
F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme. |
| immunogen |
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purified c subunit from Spinacia oleracea P69447 |
| antibody format |
|
rabbit; |
polyclonal; |
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serum; |
lyophilized |
|
| quantity |
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100 µl |
- for reconstitution add 100 µl of sterile water |
|
| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
Western blot (WB) |
| related products |
|
AS08 370 | anti-ATP synthase whole enzyme AS08 304 | anti-ATP synthase subunit alpha AS05 085 | anti-ATP synthase subunit beta AS08 312 | anti-ATP synthase subunit gamma antibody |
| additional information |
|
to be added when available |
application information
|
| recommended dilution |
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1: 1000 - 1: 10 000 with standard ECL (WB) |
| expected | apparent MW |
|
8 kDa (for Arabidopsis thaliana) |
| confirmed reactivity |
|
Arabidopsis thaliana, Nicotiana benthamina, Spinacia oleracea, Thermosynechococcus elongatus |
| predicted reactivity |
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dicots including Glycine max, Pisum sativum, Vitis vinifera, monocolts indlucing Oryza sativa, Zea mays, trees: Populus alba, Pinus thunbergii, moss: Physcomitrella patens, green algae |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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please note that increased incubation at 95ºC (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e.g. 5 or 6 subunits) may occur |
| selected references |
|
to be added when available |
| application example
0.7 μg of purified ATP-synthase complex from Spinacia oleracea (CF0F1) (1), 15.3 µg of purified ATP-synthase complex (CF0F1) from Nicotiana benthamiana (2) and 48.6 µg of Thermosynechococcus elongatus thylakoid preparation (3) were separated on 12% polyacrylamide gel and blotted on PVDF membrane. Filters where blocked (0.5h), incubated with 1: 1000 anti-AtpH antibodies (1h), followed by incubation with 1: 5 000 secondary anti-rabbit antibody (1.25h), coupled to HRP and visualized with standard ECL. | | | |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menu bar or contact us at support@agrisera.com
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