Plant/Algal cell antibodies / Populus sp.

product information

F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.

purified c subunit from Spinacia oleracea P69447

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Western blot (WB)

AS08 370 | anti-ATP synthase whole enzyme

AS08 304 | anti-ATP synthase subunit alpha

AS05 085 | anti-ATP synthase subunit beta

AS08 312 | anti-ATP synthase subunit gamma antibody

to be added when available

application information

1: 1000 - 1: 10 000 with standard ECL (WB)

8 kDa (for Arabidopsis thaliana)

Arabidopsis thaliana, Nicotiana benthamina, Spinacia oleracea,  Thermosynechococcus elongatus

dicots including Glycine max, Pisum sativum, Vitis vinifera, monocolts indlucing Oryza sativa, Zea mays, trees: Populus alba, Pinus thunbergii, moss: Physcomitrella patens,  green algae

no confirmed exceptions from predicted reactivity known in the moment

please note that increased incubation at 95ºC (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e.g. 5 or 6 subunits) may occur

Lawrence et al. (2010). Recombinant production and purification of the subunit c of chloroplast ATP synthase. Protein Expression and Purification 76: 15-24.