Plant/Algal cell antibodies / Mitochondria | Respiration

product information

Beta-CA1, beta-CA2 is a low-CO2-induced  mitochondrial carbonic anhydrase. that catalyze the reversible interconversion of carbon dioxide and water to bicarbonate and protons. Synonymes: MtCA.

recombinant Chlamydomonas reinhardtii mitochondrial CA, as described in Villand et al. 1997. Accession number Q39590 and Q39589

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

antibody is recognizing both isoforms, beta- CA1 and beta-CA2 and can be used as mitochondrial marker for low carbon dioxide grown cells of Chlamydomonas reinhardtii.

application information

1 : 1000 with standard ECL (WB)

23.7 | 21-22 kDa

no confirmed exceptions from predicted reactivity are currently known

to be added when available

Eriksson et al. (1996). Discovery of an algal mitochondrial carbonic anhydrase: Molecular cloning and characterization of a low-CO2-induced polypeptide in Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA. 93:12031-12034.

Eriksson et al. (1998). Induction and Regulation of Expression of a Low-CO2-Induced Mitochondrial Carbonic Anhydrase in Chlamydomonas reinhardtii. Plant Physiol. 116: 637-641.

Renberg et al. (2010). A Metabolomic Approach to Study Major Metabolite Changes during Acclimation to Limiting CO2 in Chlamydomonas reinhardtii. Plant physiol. 154: 187-196.