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product information
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| background |
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Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. |
| immunogen |
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KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160, monocots including Oryza sativa P04373 and Physcomitrella patens Q1XGA9 |
| antibody format |
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hen |
polyclonal |
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total IgY in PBS pH 8.0+ 0.02% sodium azide |
liquid |
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| quantity |
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| storage |
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store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS04 053 COXII | cytochrome oxidase subunit II, plant (rabbit) AS04 53A COXII | cytochrome oxidase subunit II, plant (affinity purified) AS06 151 COXIIb | cytochrome oxidase subunit IIb ( Chlamydomonas) |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1:1000 with standard ECL (WB) |
| expected | apparent MW |
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29.4 | 30 kDa (for Arabidopsis thaliana) |
| confirmed reactivity |
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Arabidopsis thaliana (leaf extract and isolated mitochondria), Brassica oleracea, Plantago major, Plantago euryphylla, Physcomitrella patens, Silene uniflora, Silene dioica |
| predicted reactivity |
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eudicots, monocots |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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not available at the moment |
| selected references |
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not available at the moment |
||| For applications or usage on species others than stated above Agrisera offer a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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