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SPS | Sucrose phosphate synthase (maize)

265 €
Buy 2 items of this product for 198 €/each
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AS06 185   |  clonality: polyclonal  |  host: rabbit  |  reactivity: Z.mays, S.lycopersicum   | cellular [compartment marker] of cytoplasm

PRODUCT INFORMATION IN PDF

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Item No:
AS06 185

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product information
Background

SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.

Immunogen

Synthetic peptide derived from Zea mays SPS protein sequence (P31927).

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications
Western Blot (WB), Immunolocalization (IL)
Related products
AS03 035A | anti-SPS | Sucrose phosphate synthase, global
AS03 035-HRP | anti-SPS | Sucrose phosphate synthase, global (40 µg, HRP-conjugated)
AS03 035-ALP | anti-SPS | Sucrose phosphate synthase, global (40 µg, ALP-conjugated)
AS15 2995 | anti-SPSA1 | Sucrose phosphate synthase isoform A1, rabbit antibody
AS15 2996 | anti-SPSA2 | Sucrose phosphate synthase isoform A1, rabbit antibody
AS15 2997 | anti-SPSC | Sucrose phosphate synthase isoform C, rabbit antibody

Plant and algal protein extraction buffer
Additional information

Total IgG concentration is 3 µg/µl

application information
Recommended dilution

1 : 2 000 with ECL (WB), 1: 1500 (IL)

Expected | apparent MW

120 | ~130 for Zea mays

Confirmed reactivity

Solanum lycopersicum, Zea mays

Predicted reactivity

monocots: Saccharum officinarum, Triticum aestivum and Oryza sativa

Not reactive in

Hordeum vulgare

Additional information

To be added when available

Selected references Seger et al. (2014). Impact of concurrent overexpression of cytosolic glutamine synthetase (GS 1 ) and sucrose phosphate synthase (SPS) on growth and development in transgenic tobacco. Planta. 2014 Sep 12.
Rounis et al. (2014). Seeded and Parthenocarpic Cherry Tomato Fruits Exhibit Similar Sucrose, Glucose, and Fructose Levels, Despite Dissimilarities in UGPase and SPS Gene Expression and Enzyme Activity. J Plant Growth Regul. DOI 10.1007/s00344-014-9441-1. (immunolocalization)

Application example

10 µg of total leaf protein from (1) A.thaliana, (3) Zea mays and (4) Hordeum vulgare extracted with PEB (AS08 300) as well as 10 µg cytosolic protein from (2) A.thaliana were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1.5h (30V) to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-SPS (AS06 0185, 1:2000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder.  Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (90s, high sensitivity).

 

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