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FIE | Fertilization-independent endosperm

195 €

AS12 2616 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

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AS12 2616

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product information
Background FIE (fertilization-independent endosperm) belongs to polycomb group (PcG) protein which act by forming multiprotein complexes, required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. 
Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana FIE protein sequence, UniProt: Q9LT47, TAIR: At3g20740

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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collection of antibodies to proteins involved in plant development

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

41 kDa

Confirmed reactivity Recombinant FIE protein
Predicted reactivity Arabidopsis thaliana, Triticum aestivum
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

This antibody so far has been confirmed to detect recombinant FIE protein, GFP-tagged. 

Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous FIE.

Selected references

to be added when available, antibody released in March 2016. 


 


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