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PR-3 / CHN | Class I chitinase

345 €

AS07 207  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. stolonifera cv. ‘Penncross’C. annuum, N. tabacum, P. abies, S. esculentum, S. lycopersicum, S. tuberosum, V. vinifera | Cellular marker of vacuolar contents

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AS07 207

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product information
Background

Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known.  Glucanase I  (PR-2) and chitinase I (PR-3) contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis.

Immunogen

Purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al., 1990; van Buuren et al., 1992): 1) Chitinase A (CHN A) P08252 encoded by gene chn48 derived from the N. tomentosiformis ancestor of tobacco. 2) Chitinase B (CHN B) P24091 encoded by gene chn50 derived from the N. sylvestris ancestor of tobacco.

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 2 mg
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Co-immunoprecipitation (Co-IP), Immunolocalization (IL), Western Blot (WB)
Related products

AS10 687 | PR-1 | Pathogenesis-related protein 1, rabbit antibodies

AS12 2366 | PR-2 | pathogenesis-related protein 2, rabbit antibodies for Arabidopsis thaliana

AS07 208 | PR-2 | GLU I | class I beta-1,3-glucanase, rabbit antibodies for other species, not Arabidopsis thaliana

AS12 2369 | PR-4 | Pathogenesis-related protein 4, rabbit antibodies

AS12 2373 | PR-5 | Pathogenesis-related protein 5, rabbit antibodies

collection of antibodies to other proteins involved in a response to pathogen attack

Secondary antibodies

Additional information
Antibody is recognizing closely related tobacco class I isoforms: endochitinase A CHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa)
This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51. 
application information
Recommended dilution

8 ug/ml (WB)

Expected | apparent MW

35, 34 | 32 and 34 kDa

Confirmed reactivity Agostis stolonifera cv. ‘Penncross’, Capsicum annuum, Nicotiana tabacum, Picea abies, Solanum esculentum, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
Predicted reactivity

Arabidopsis thaliana, Zea mays

Not reactive in

No known expection is know at this time

Additional information

Important note: For blocking 5 % skim milk in PBS without Ca++ should be used.

This antibody is purified by affinity chromarography on Portein G.

Selected references Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)
Ko et al. (2016). Constitutive expression of a fungus-inducible carboxylesterase improves disease resistance in transgenic pepper plants. Planta. 2016 Aug; 244(2):379-92. doi: 10.1007/s00425-016-2514-6. Epub 2016 Apr 13.
Anil Kumar et al. (2016). Beyond just being foot soldiers – osmotin like protein (OLP) and chitinase (Chi11) genes act as sentinels to confront salt, drought, and fungal stress tolerance in tomato. Environmental and Experimental Botany 132 (2016) 53–65
Wu et al. (2016). Laminarin modulates the chloroplast antioxidant system to enhance abiotic stress tolerance partially through the regulation of the defensin-like gene expression. Plant Science, Volume 247, June 2016, Pages 83–92.
Falcioni et al. (2013). Effect of salicylic acid treatment on tomato plant physiology and tolerance to potato virus X infection. Eur J Plant Pathol DOI 10.1007/s10658-013-0333-1.
Munger et al. (2012). Beneficial 'unintended effects' of cereal cystatin in transgenic lines of potato, Solanum tuberosum. BMC Plant Biology, 12:198.
Sticher et al. (1993). Posttranslational processing of a new class of hydroxyproline-containing proteins: Prolyl hydroxylation and C-terminal cleavage of tobacco (Nicotiana tabacum) vacuolar chitinase. Plant Physiol. 101, 1239-1247.

Application example

 

 

 

Detection of tobacco chitinase I in ng loaded per respective well using anti-tobacco chitinase I antibodies. Primary antibodies have been used at 8 µg/ml.


 


 western blot detection using class I chitinase antibodies

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