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GSNOR | S-nitrosoglutathione reductase

272 €
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AS09 647  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Helianthus annuus

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AS09 647

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product information
Background

S-nitrosoglutathione reductase (GSNOR) is a cytoplasm localized enzyme which plays a key role in formaldehyde detoxification and is down regulated by wounding and activated by salicylic acid (SA).
Alternative protein names: Alcohol dehydrogenase class-3, Alcohol dehydrogenase class-III, FALDH, FDH formaldehyde dehydrogenase, alcohol dehydrogenase III, HOT5, S-(hydroxymethyl)glutathione dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH

Immunogen

Overexpressed, full length GSNOR derived from Arabidopsis thaliana Q96533, At5g43940

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

Collection of antibodies to other proteins involved in stress response

Additional information

Antibody is easily detecting GSNOR in a load per well of 5 µg of total Arabidopsis cell extract

application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

40.7 | 40.7 kDa

Confirmed reactivity Arabidopsis thaliana, Helianthus annuus
Predicted reactivity Brassica napus, Oryza sativa, Pisum sativum, Populus balsamifera, Ricinus communis, Solanum tuberosum, Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Jain et al. (2018). S-nitrosylation/denitrosylation as a regulatory mechanism of salt stress sensing in sunflower seedlings. Physiol Plant. 2018 Jan;162(1):49-72. doi: 10.1111/ppl.12641.
Kovacs et al. (2016). ROS-Mediated Inhibition of S-nitrosoglutathione Reductase Contributes to the Activation of Anti-oxidative Mechanisms.Front. Plant Sci., 10 November 2016
Zhou et al. (2016). Arabidopsis CaM1 and CaM4 Promote Nitric Oxide Production and Salt Resistance by Inhibiting S-Nitrosoglutathione Reductase via Direct Binding. PLoS Genet. 2016 Sep 29;12(9):e1006255. doi: 10.1371/journal.pgen.1006255. eCollection 2016.
Lee et al. (2008). Modulation of nitrosative stress by S-nitrosoglutathione reductase is critical for thermotolerance and plant growth in Arabidopsis. The Plant Cell 20: 786-802.

Application example

Western blot using anti-GNSOR antibody
2 µg of total protein from (1) Arabidopsis thaliana WT and GSNOR null mutant were separated on 7.5%SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubatedanti-GSNOR antibodies at a dilution of 1: 1 000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000).

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