CCS | Chloroplastic copper chaperone for SOD

345 €

AS07 219 |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana,  B. juncea, Z. mays, O. sativa  


27 st
Item No:
AS07 219

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product information

Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD.


The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5,  fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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AS09 524 | anti-MnSOD | manganese superoxide dismutase

collection of antibodies to plant stress proteins

Additional information
application information
Recommended dilution

1 : 1000 with alkaline phosphatase (WB)

Expected | apparent MW

33.8 | 37 kDa (Arabidopsis thaliana)

Confirmed reactivity

Arabidopsis thaliana, Brassica juncea, Oryza sativa,Zea mays

Predicted reactivity

dicots including: Glycine max, Solanum tuberosum

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references Speth et al. (2013). RACK1 scaffold proteins influence miRNA abundance in Arabidopsis. Plant J. Aug 13.

Abdel-Ghany et al (2005). .AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7.  FEBS Lett. 11:2307-12.

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