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DnaK2 | Heat shock protein 70-2 (HSP70-2)

345 €

AS08 350  |  clonality: polyclonal  |  host: rabbit  |  reactivity: cyanobacteria

PRODUCT INFORMATION IN PDF

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Item No:
AS08 350

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product information
Background

DnaK2 belongs to the family of heat shock proteins 70 and functions as a chaperone involved in stress response. Dnak2 is heat induced but also present at high level under normal growth conditions.

Immunogen

full length recombinant DnaK2 protein derived from Synechocystis PCC 6803 DnaK2 protein sequence P22358

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS07 270 | anti-DnaK rabbit antibody

Additional information

This product can be sold containing proclin if requested

application information
Recommended dilution

1: 1000 (WB)

Expected | apparent MW

67.6 | 76 kDa

Confirmed reactivity Synechocystis PCC 6803
Predicted reactivity

Anabaena sp., Crocosphaera watsonii, Cyanothece sp. PCC 7424, Lyngbya sp. PCC 8106, Microcoleus chthonoplastes PCC 7420,  Nodularia spumigena CCY 9414, Nostoc sp.,  Synechococcus sp. PCC 7942 and other cyanobacterial strains

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

not available at the moment

Selected references

not available yet, antibody relased in February 2009


application example

 

 

10 µg of total protein from total cell extract of Synechocystis PCC 6803 wilde type (WT) and mutant strain that lacks Hsp16.6 were separated on  8 % gel and blotted on nitrocellulose membrane. Filters were blocked (1h), incubated with 1:1000 anti-DnaK2 antibodies (2h) followed by incubation with 1: 2500 secondary anti-rabbit (1h) coupled to HRP and visualization with standard ECL plus (GE Healthcare), exposure time 10 seconds.

 

 

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