HSP17.6 | Cytosolic class I heat shock protein 17.6
AS08 284 | clonality: polyclonal | host: hen | reactivity: Arabidopsis thaliana
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1: 1000 with standard ECL (WB)
|Expected | apparent MW||
|Not reactive in||
no confirmed exceptions from predicted reactivity known at the moment
There are six total class I genes. Essentially this antibody might react to some extent with all of them. But does not react with class II, organelle, or any other shsp classes.
|Selected references||Mao and Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244.|
10 µg of total protein from (C) Arabidopsis thaliana WT, non-stressed, (HS) Arabidopsis thaliana WT stressed at 38ºC were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody (1: 10 000 from Abcam).
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