HSP16.9 | 16.9 kDa class I heat shock protein 2
AS12 2570 | clonality: polyclonal | host: rabbit | reactivity: Triticum aestivum
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1: 1000 - 1:10 000 with standard ECL (WB)
|Expected | apparent MW||
|Predicted reactivity||Aegilops kotschyi, Triticum sp., Hordeum vulgare
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
to be added when available, antibody released in June 2013.
From 0.1-200 ng of recombinant HSP16B protein from Triticum aestivum were separated on 15 % SDS-PAGE and blotted 1h to nitrocellulose using semi-dry transfer at 15 V for 1 hour in a buffer containing: 48mM Tris, 39 mM Glycine, 0.02% SDS, 20 % methanol, pH 9.2. Blots were blocked with with 2.5% milk in TBS (50mM Tris, 150 mM NaCl, pH 7.5) for 1 hour at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 2h at RT TBS with 2.5% milk with agitation. Three washes (15 minutes each) were carried out with TBS and 0.05% Tween20 at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed using Pierce western blotting substrate (#32106). The exposure time used for visualization was 6.5 minutes.
Courtesy of Indu Santhanagopalan, Dept. of Biochem and Mol Biology, Lederle Graduate Research Center, USA.
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