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PPI | papain

218 €

AS09 545 | clonality: polyclonal | host: rabbit | reactivity: Carica papaya

PRODUCT INFORMATION IN PDF

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Item No:
AS09 545

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product information
Background

Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans. Alternative names: Papaya proteinase I, allergen= Car p 1.

Immunogen

native papain isolated and purified from Carica papaya

Host Rabbit
Clonality Polyclonal
Clone
Purity Purified IgG
Format Lyophilized in phosphate buffer saline
Quantity 10 mg
Reconstitution For reconstitution please add 1 ml of sterile destilled water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
Related products

AS09 546 | anti-PPI | papain (biotinylated)

AS09 547 | anti-PPI | papain (affinity purified)

Plant protein extraction buffer

Secondary antibodies

Additional information

antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

application information
Recommended dilution

1: 1000 - 1: 100 000 for techniques listed above

Expected | apparent MW

38.9 kDa

Confirmed reactivity Carica papaya
Predicted reactivity

Carica papaya

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Selected references

to be added when available



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