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PEPC | phosphoenolpyruvate carboxylase (40 ĩg, HRP-conjugated)

406 €

AS09 458-HRP | clonality:polyclonal | host:rabbit | reactivity: A. comosus, A. thaliana, S. PCC 6803, Ch. velia, H. vulgare, J. curcas, Lupinus sp. , M. crystallinum, N. tabacum, O. sativa, Saccharum spp. hybrid clone C91-301, S. laricifolia, Z. mays, Synechocystis sp. , P. tricornutum (strain CCAP 1055/1), T. weissfloggi

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AS09 458-HRP

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product information
Background

PEPC (phosphoenolpyruvate carboxylase), EC=4.1.1.31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate. Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3

Immunogen

KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0, At1g53310 (PEPC 1), Q84VW9, At3g14940 (PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzyme NP_177043.2 (PEPC 4)

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Liquid in PBS pH 7.4, conjugated to HRP
Quantity 40 µg
Reconstitution
Storage

Store at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Tested applications western blot (WB)
Related products

AS07 241 | anti-PEPCK | PEP carboxy kinase

Additional information
application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

110 | 105 kDa

Confirmed reactivity

Ananas comosus, Arabidopsis thaliana, Synechocystis PCC 6803, Chromera velia, Hordeum vulgare, Jatropha curcas, Lupinus sp. , Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Saccharum spp. hybrid clone C91-301, Salsola laricifolia, Zea mays, Synechocystis sp. , Phaeodactylum tricornutum (strain CCAP 1055/1), Thalassiosira weissfloggi

Predicted reactivity Cucumis sativus (PEPC1,PEPC2,PEPC3), Flaveria trinervia, Glycine max, Lupinus albus, Mammillaria thornberi, Manihot obovata, Medicago sativa, Morinda citrifolia, Nopalea gaumeri, Opuntia macbridei, Pachycereus pringlei, Solanum tuberosum, Spinacia oleracea, Streptanthus tortuosus, Pachycereus hollianus, Pisum sativa, Phaseolus vulgaris,Triticum aestivum,  algae, diatoms: Thalassiosira pseudonana, other species: Salmonella sp., Schiedea hookeri, Shigella sp. Schiedea sarmentosa, Streptanthus farnsworthianus, Tacinga saxatilis,Yersinia sp. Vibrio sp.
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information Antibody can be also used following 2D gel electrophoresis.
Selected references


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