ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

RPS14 | 40S ribosomal protein S14-1

361 €

AS12 2111 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, Z. mays, Ch. reinhardtii

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
30 st
Delivery:
Shipping:
Item No:
AS12 2111

Info: Product suggestions Add review
product information
Background

 40S ribosomal protein S14-1 is localized in cytoplasm and belongs to ribosomal protein S11P family.

Immunogen

KLH-conjugated synthetic peptide derived from a C-terminal of Arabidopsis thaliana  Q9SIH0

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified in PBS pH 7.4.
Format
Quantity 100 µg
Reconstitution
Storage

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS13 2650 | anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies
AS09 478| anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies
AS08 331| anti-L-30 | 50S ribosomal protein L30, rabbit antibodies
AS11 1738| anti-Rpl1 | 50S ribosomal protein L1, rabbit antibodies
AS15 2876 | anti-RPL2 | ribosomal protein L2 (chloroplastic), rabbit antibodies
AS14 2780 | anti-RPL33 | 50S ribosomal protein L33, chloroplastic, rabbit antibodies
AS14 2781 | anti-RPL36 | 50S ribosomal protein L36, chloroplastic, rabbit antibodies
AS12 2115 | anti-RPL37 | ribosomal protein L37, cytoplasmic, rabbit antibodies
AS15 2875 | anti-RPS1 | ribosomal protein S1 (chloroplastic), rabbit antibodies
AS12 2114 | anti-RPS12 | ribosomal protein S12, chloroplastic, rabbit antibodies
AS14 2779 | anti-RPS15 | 30S ribosomal protein S15, chloroplastic, rabbit antibodies
AS10 719 | anti-Rps2 | anti-ribosomal subunit 2 (cytoplasmic), rabbit antibodies
AS08 309 | anti-S1 | anti-30S ribosomal protein S1, rabbit antibodies
AS15 3068 | anti-S4 |   mitochondrial ribosomal small subunit protein S4, rabbit antibodies
AS15 3067 | anti-S10 |  mitochondrial ribosomal small subunit protein S10, rabbit antibodies
AS15 3069 | anti-L16   mitochondrial ribosomal large subunit protein L16, rabbit antibodies

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 1000 with standard ECL (WB)

Expected | apparent MW

16 | 15 kDa

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii, Zea mays
Predicted reactivity

Brassica napus, Fusarium oxysporum, Lupinus luteus, Ostreococcus tauri, Oryza sativa, Picea sitchensis, Populus trichocarpa,Sorghum bicolor, Ricinus communis 

other species: chicken, human, mouse, rat, salmon, Candidia albicans, Trypanosoma brucei,

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information 20 µg of total protein is needed for detection of S14 in Arabidopsis thaliana
Selected references Linster et al. (2015). Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis. Nat Commun. 2015 Jul 17;6:7640. doi: 10.1038/ncomms8640.

application example

western blot detection of S14 protein in Arabidopsis

20 µg of total protein from Arabidopsis thaliana extracts from (1) total cell, nuclei (2), chloroplasts (3), thylakoids (4) were extracted with preparation buffer (330 mM sorbitol, 25mM Tricine pH 7.8, 1mM EDTA, 10mM KCl, 0,15% BSA, 4mM Na ascorbat and 7mM L-cysteine) and separated on 12 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and blotted 1h 30min to Immobilon™PVDF (Millipore) membrane. Blots were blocked with 10% dry milk in Tris-buffered saline TBS-T (50 mM tris, 150 mM NaCl, pH 7.6 + 1ML 20% TWEEN 20) for 1h at room temperature (RT) with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2 000 (in TBS-T) overnight at 4ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Pierce) diluted to 1:20 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ThermoSuper Signal WestPico according to the manufacturers instructions. Exposure time was 3 minutes. Courtesy of Dr. Rikard Fristedt, UCLA, USA

 

western blot detection of S14 in various species

 

5 µg of total protein from Arabidopsis thaliana (1), Zea mays (2), Chlamydomonas reinhardtii (3), Salmo salar (4)  extracted with PEB  were separated on 4-12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 2 minutes.


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com