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PIP1;1, PIP1;2, PIP1;3 | Aquaporins

454 €

AS09 489 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Raphanus sativus

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AS09 489

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product information
Background

PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane. PIP1;2 is also designated as PIP1B.

Immunogen

KLH-conjugated synthetic peptide derived from N terminus of Raphanus sativus PAQ1O80368. Chosen peptide is conserved in PIP1;1, PIP1;2, PIP1;3 N-terminus.

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.For long time storage over 1 year please dilute the antibody solution 10 fold with 10 mM Tris-buffered saline containing 10% glycerol. Glycerol may suppress denaturation of protein.
Tested applications ELISA (ELISA), Western blot (WB)
Related products

collection of antibodies to plasma membrane proteins

AS09 488 | anti-PIP2;1 | aquaporin PIP2;1

AS09 490 | anti-PIP2;2 | plasma membrane aquaporin 2b

AS09 491 | anti-PIP2;1,PIP2;2,PIP2;3 |plasma membrane intrinistic protein 2-1,2-2, 2-3

Plant protein extraction buffer

Secondary antibodies

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protocol for plant membrane isolation can be found here.

application information
Recommended dilution 1 : 8000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW

30.66 | 28 kDa (Raphanus sativus)

Confirmed reactivity Arabidopsis thaliana, Nicotiana tabacum, Raphanus sativus, Zea mays
Predicted reactivity Lupinus sp. , Hordeum vulgare, Solanu lycopersicum, Ricinus communis, Oryza sativa, Populus trichocarpa
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Antibody reacts also with PIP2 isoforms in Zea mays.

Manufactured by Operon Biotechnologies.

Selected references Pengelly et al. (2014). Transplastomic integration of a cyanobacterial bicarbonate transporter into tobacco chloroplasts. J Exp. Botany, doi:10.1093/jxb/eru156.

application example

 

 

1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana  were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIP1s ntibodies (diluted in 1: 500 (left panel) and 1: 1000 (right panel), AS09 489, and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

 

  western blot using anti-PIP1s antibodies

 


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