Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 3
AS08 351 | clonality: polyclonal | host: rabbit | reactivity: A.thaliana, N. tabacum, P. patens, P. sativum, S. oleracea, T. salsuginea, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus sp.
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1: 2000 - 1: 25 000, 1: 100 000 (WB), 1: 500 (FACS). 1: 100 (IL)
|Expected | apparent MW||
88 | 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
|Confirmed reactivity||Arabidopsis thaliana, Nicotiana tabacum, Physcomitrella patens, Pisum sativum, Spinacia oleracea, Thellungiella salsuginea, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus sp.|
Aegilops tauschii, Ananas comosus, Anthurium amnicola, Capsicum annuum, Catalpa bungei, Cicer arietinum, Cucumis melo, Glycine soja, Gossypium arboreum, Medicago truncatula, Morus notabilis, Nelumbo nucifera, Nicotiana sylvestris, Nicotiana tabacum, Noccaea caerulescens, Populus trichocarpa, Ricinus communis, Theobroma cacao, Zostera marina, Vigna radiata, Vitis vinifera
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
antibody detects Toc75 POTRA domain 3 as purified protein, in chloroplast fraction and in crude envelope fraction
|Selected references||Sasaki and Yamamoto (2015). Arabidopsis LAZY1 is a peripheral membrane protein of which the carboxy-terminal fragment potentially interacts with microtubules. Copyright © 2015 The Japanese Society for Plant Cell and Molecular Biology, Plant Biotechnology, 32, 1–6 (2015) DOI: 10.5511/plantbiotechnology.15.0116a
Hsueh et al. (2014). The chloroplast outer envelope protein P39 in Arabidopsis thaliana belongs to the Omp85 protein family. Proteins. 2014 Nov 17. doi: 10.1002/prot.24725.
Vera-Estrella et al. (2014). Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics. 2014 Jun 2. pii: S1874-3919(14)00288-7. doi: 10.1016/j.jprot.2014.05.018.
580 ng of Chl of Pisum sativum plants (10 day old) (2, 4) and 10 µg of combined envelopes of Pisum sativum 10 day-old (1,3) were separated on 15% SDS-PAGE and blotted 2h to PVDF. PVDF was blocked 1h with 3% non-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Toc75 POTRA domain 3 antibodies AS08 351 (1:2000 and 1: 5000, 1h) and secondary donkey-anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 3% non fat milk powder. Antibody incubations were followed by washings in TBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with HRP substrate Peroxide solution & luminol detection reagent according to the manufacturers instructions (Millipore). Exposure time was 600 seconds.
Ashita Dave and Barry D. Bruce (UTK)
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