Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 3
AS08 351 | clonality: polyclonal | host: rabbit | reactivity: A.thaliana, N. tabacum, P. sativum, S. oleracea, T. salsuginea, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus sp.
|Info:||Product suggestions||Read reviews|
1: 2000 - 1: 25 000, 1: 100 000 (WB), 1: 500 (FACS). 1: 100 (IL)
|Expected | apparent MW||
88 | 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
Arabidopsis thaliana, Nicotiana tabacum, Pisum sativum, Spinacia oleracea, Thellungiella salsuginea, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus sp.
Ricinus communis, Vitis vinifera, Populus trichocarpa
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
antibody detects Toc75 POTRA domain 3 as purified protein, in chloroplast fraction and in crude envelope fraction
|Selected references||Sasaki and Yamamoto (2015). Arabidopsis LAZY1 is a peripheral membrane protein of which the carboxy-terminal fragment potentially interacts with microtubules. Copyright © 2015 The Japanese Society for Plant Cell and Molecular Biology, Plant Biotechnology, 32, 1–6 (2015) DOI: 10.5511/plantbiotechnology.15.0116a
Hsueh et al. (2014). The chloroplast outer envelope protein P39 in Arabidopsis thaliana belongs to the Omp85 protein family. Proteins. 2014 Nov 17. doi: 10.1002/prot.24725.
Vera-Estrella et al. (2014). Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics. 2014 Jun 2. pii: S1874-3919(14)00288-7. doi: 10.1016/j.jprot.2014.05.018.
580 ng of Chl of Pisum sativum plants (10 day old) (2, 4) and 10 µg of combined envelopes of Pisum sativum 10 day-old (1,3) were separated on 15% SDS-PAGE and blotted 2h to PVDF. PVDF was blocked 1h with 3% non-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Toc75 POTRA domain 3 antibodies AS08 351 (1:2000 and 1: 5000, 1h) and secondary donkey-anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 3% non fat milk powder. Antibody incubations were followed by washings in TBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with HRP substrate Peroxide solution & luminol detection reagent according to the manufacturers instructions (Millipore). Exposure time was 600 seconds.
Ashita Dave and Barry D. Bruce (UTK)
||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at firstname.lastname@example.org