Rubisco | 557 kDa hexadecamer

332 €

AS07 218  |  serum  |  clonality: polyclonal  |  host: rabbit  |  reactivity:  plants and green algae


14 st
Item No:
AS07 218

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product information

Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.


purified 557 kDa hexadecamer Rubisco protein complex from Spinacia oleracea (SIGMA-ALDRICH R-8000), UniProt: P00875 and Q43832

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Western blot (WB)
Related products

AS03 037 | anti-RbcL | Rubisco large subunit, form I and form II (50 µl)
AS03 037A | anti-RbcL | Rubisco large subunit, form I and form II (50 µg affinity purified)

AS03 037-HRP| anti-RbcL | Rubisco large subunit, form I and form II (40 µg, HRP-conjugated)

AS15 2955
| anti-RbcL II | Rubisco large subunit, form II (50 µl), rabbit antibody
AS15 2955S | RbcL II | Rubisco form II positive control/quantitation standard

AS01 017  | anti-RbcL | Rubisco large subunit, form I, chicken antibody
AS01 017S | Rubisco protein standard for quantitative western blot or positive control

AS03 037PRE | Rubisco large subunit, pre-immune serum
AS09 409 | Rubisco quantitation kit
AS15 2994 | Rubisco ELISA quantitation kit 

AS07 259 | anti-RbcS | Rubisco small subunit (SSU), rabbit antibody
AS07 222
| anti-RbcS | Rubisco small subunit (SSU) from pea, rabbit antibody

matching secondary antibody

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 10 000-1 : 20 000 on 0.5-10 ug total cellular protein/lane and standard (WB). 1 : 500-1:1000 (IL)
Expected | apparent MW

53-55 | 53-55 kDa

Confirmed reactivity Arabidopsis thaliana, Aucuba japonica, Chlamydomonas reinhardtii, Glycine max, Manihot esculenta Crantz, Physcomitrella patens, Pisum sativum, Populus sp., Salsola laricifolia, Solanum tuberosum, Spinacia oleracea, Synechocystis sp. PCC 6803, Synechococcus sp. PCC7942, Zea mays
Predicted reactivity Begonia sp., Daucus carota
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

RbcS subunit is not detected by this antibody

This product can be sold containing proclin if requested

Selected references Zhang et al. (2017). Composition of photosynthetic pigments and photosynthetic characteristics in green and yellow sectors of the variegated Aucuba japonica ‘Variegata’ leaves. Flora, Vol 240, March 2018, Pages 25–33.
Wang et al. (2017). Re-creation of a Key Step in the Evolutionary Switch from C3 to C4 Leaf Anatomy. Curr Biol. 2017 Nov 6;27(21):3278-3287.e6. doi: 10.1016/j.cub.2017.09.040.
Wen and Zhang (2014). Salsola laricifolia, another C 3 –C 4 intermediate species in tribe Salsoleae s.l. (Chenopodiaceae). Photosynth Res. 2014 Sep 17. (immunolocalization)
Feifei et al. (2014). Comparison of Leaf Proteomes of Cassava (Manihot esculenta Crantz) Cultivar NZ199 Diploid and Autotetraploid Genotypes. PLoS One. 2014 Apr 11;9(4):e85991. doi: 10.1371/journal.pone.0085991. eCollection 2014.

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