AtpA | Alpha subunit of ATP synthase, chloroplastic
AS08 304 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. modesticaldum, Z. mays
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|Recommended dilution||1 : 5 000 (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana, Capsicum annuum, Heliobacterium modesticaldum, Zea mays|
|Predicted reactivity||Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Cyanobacteria|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Selected references||Jeon et al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y.
Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016.
Camejo et al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370.
Yang et al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006.
Lane 1: Arabidopsis thaliana total leaf protein extract;
Lane 2: Zea mays total leaf protein extract
5 µg of total protein were loaded per each lane.
Proteins were separated in a 12% or 5-15% gradient gel following by a transfer to nitrocellulose membrane. Membrane was blocked with TBST + 4% non-fat dried milk, 20 min following by three washes in TBST. Incubation time with primary and secondary antibodies was 1 hr primary, 30 min for secondary antibodies. Manufacturer of secondary antibodies: Bio-Rad
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