Lhcb6 | CP24 chlorphyll a/b-binding protein of plant PSII

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AS01 010  |  clonality: polyclonal  | host: rabbit  |  reactivity: Arabidopsis thaliana


35 st
Item No:
AS01 010

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product information

Lhcb6 is one of the 3 highly conserved minor chlorophyll a/b-binding proteins exclusively associated with Photosystem II in plants and algae. Together with Lhcb4 and Lhcb5, it regulates the energy flow from the outer antenna to the reaction center through the action of the xanthophyll cycle.


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Lhcb6, UniProt: Q9LMQ2, TAIR:At1g15820. This sequence is highly conserved in angiosperms (monocots and dicots) and gymnosperms.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes
Tested applications Western blot (WB)
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Available antibodies against pigment-binding proteins - LHC recommended secondary antibody
Additional information
application information
Recommended dilution 1 : 1000-1 : 5000 (WB)
Expected | apparent MW

27.5 | 24 kDa for Arabidopsis thaliana

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity

Dictos, Gymnosperms, Hordeum vulgare, Physcomitrella patens, Pisum sativum, Selaginella martensii, Spinacia oleracea, Zea may 

Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Protein is processed into mature form (Jansson 1999).

This antibody is a re-make of former Lhcb6 antibody from Agrisera and is made to the same peptide. 
Selected references To be added when available, antibody released in May 2017.

Application example

western blot using anti-Lhcb6 antibodies

5 µg of total protein from Arabidopsis thaliana extracted with Agrisera Protein Extraction Buffer PEB (AS08 300) and denatured in PEB at 70°C for 5 min. were separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry or tank transfer (blotted 15h to PVDF using tank-transfer - 30V). Blots were blocked with TBST with 4 % BSA for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in TBS-T with 2% BSA. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1: 50 000 in for 1h at RT with agitation in TBS-T with 2% BSA. The blot was washed as above and developed for Clarity Western ECL Substrate Bio-Rad for 5 minutes. Exposure time was 25 seconds.

Courtesy of Dr. Robert Luciński, Department of Biology, UAM, Poznań, Poland

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