Lhcb6 | CP24 chlorphyll a/b-binding protein of plant PSII
AS01 010 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
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1: 1000 - 1 : 5000 (WB)
|Expected | apparent MW||
27.5 | 24 kDa for Arabidopsis thaliana
|Confirmed reactivity||Arabidopsis thaliana|
Hordeum vulgare, Pisum sativum, Selaginella martensii, Spinacia oleracea, Zea mays
|Not reactive in||
No confirmed exceptions from predicted reactivity known in the moment
Protein is processed into mature form (Jansson 1999).
This antibody is a re-make of former Lhcb6 antibody from Agrisera and is made to the same peptide.
|Selected references||To be added when available, antibody released in May 2017.|
5 µg of total protein from Arabidopsis thaliana extracted with Agrisera Protein Extraction Buffer PEB (AS08 300) and denatured in PEB at 70°C for 5 min. were separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry or tank transfer (blotted 15h to PVDF using tank-transfer - 30V). Blots were blocked with TBST with 4 % BSA for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in TBS-T with 2% BSA. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1: 50 000 in for 1h at RT with agitation in TBS-T with 2% BSA. The blot was washed as above and developed for Clarity Western ECL Substrate Bio-Rad for 5 minutes. Exposure time was 25 seconds.
Courtesy of Dr. Robert Luciński, Department of Biology, UAM, Poznań, Poland
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