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Lhcb6 | CP24 chlorphyll a/b-binding protein of plant PSII

265 €
Buy 2 items of this product for 198 €/each
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AS01 010  |  clonality: polyclonal  | host: rabbit  |  reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

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Item No:
AS01 010

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product information
Background

Lhcb6 is one of the 3 highly conserved minor chlorophyll a/b-binding proteins exclusively associated with Photosystem II in plants and algae. Together with Lhcb4 and Lhcb5, it regulates the energy flow from the outer antenna to the reaction center through the action of the xanthophyll cycle.

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Lhcb6, UniProt: Q9LMQ2, TAIR:At1g15820. This sequence is highly conserved in angiosperms (monocots and dicots) and gymnosperms.

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution
For reconstitution please add 50 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes

Tested applications

Western Blot (WB)

Related products
AS01 011 | A set of 10 plant anti-Lhca and anti-Lhcb antibodies

AS01 011 Chlamydomonas | A set of anti-Lhc antibodies for Chlamydmonas

Available antibodies against pigment-binding proteins - LHC recommended secondary antibody
Additional information
application information
Recommended dilution

1: 1000 - 1 : 5000 (WB)

Expected | apparent MW

27.5 | 24 kDa for Arabidopsis thaliana

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Hordeum vulgare, Pisum sativum, Selaginella martensii, Spinacia oleracea, Zea mays
angiosperms (monocots and dicots), gymnosperms, Physcomitrella patens

Not reactive in

No confirmed exceptions from predicted reactivity known in the moment

Additional information
Protein is processed into mature form (Jansson 1999).

This antibody is a re-make of former Lhcb6 antibody from Agrisera and is made to the same peptide. 
Selected references To be added when available, antibody released in May 2017.

Application example



western blot using anti-Lhcb6 antibodies


5 µg of total protein from Arabidopsis thaliana extracted with Agrisera Protein Extraction Buffer PEB (AS08 300) and denatured in PEB at 70°C for 5 min. were separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry or tank transfer (blotted 15h to PVDF using tank-transfer - 30V). Blots were blocked with TBST with 4 % BSA for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in TBS-T with 2% BSA. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1: 50 000 in for 1h at RT with agitation in TBS-T with 2% BSA. The blot was washed as above and developed for Clarity Western ECL Substrate Bio-Rad for 5 minutes. Exposure time was 25 seconds.

Courtesy of Dr. Robert Luciński, Department of Biology, UAM, Poznań, Poland

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com