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FeSOD | Chloroplastic Fe-dependent superoxide dismutase

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS06 125  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. maritima, A. thaliana, Ch. reinhardtii, D. bardawil, D.salina, O. sativa, S. tuberosum, Z. mays

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AS06 125

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product information
Background

Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD

Immunogen

overexpressed Chlamydomonas reinhardtii thioredoxine fusion protein A8IGH1, FeSOD excised from a gel piece

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS06 170 | anti-CSD2 | chloroplastic Cu/Zn superoxide dismutase

AS07 219 | anti-CCS | chloroplastic copper chaperone for SOD

AS09 524 | anti-MnSOD | manganese superoxide dismutase

Collection of antibodies to Arabidopsis proteins

Collection of antibodies to Chlamydomonas proteins

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 1500 - 1:5000 (WB)

Expected | apparent MW

25 | 22 kDa

Confirmed reactivity Armeria maritima, Arabidopsis thaliana, Chlamydomonas reinhardtii, Dunaliella bardawil, Dunaliella salina, Oryza sativa, Solanum tuberosum, Zea mays
Predicted reactivity

dicots including: Glycine max, Solanum lycopersicum, trees: Pinus pinaster, Populus balsamifera, moss: Physcomitrella patens, algae including: Dunaliella salina, Volvox carteri

Not reactive in

cyanobacteria

Additional information

The antibody will detect FeSOD enzyme only in plants grown on low Cu (0.1 μM).Reference: Salah et al (2005) Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. Plant Cell, 17, 1233-1251

This product can be sold containing ProClin if requested

Selected references Volgusheva et al. (2017). Comparative analyses of H2 photoproduction in magnesium- and sulfur-starved Chlamydomonas reinhardtii cultures. Physiol Plant. 2017 Apr 7. doi: 10.1111/ppl.12576.
Momčilović et al. (2014). Improved procedure for detection of superoxide dismutase isoforms in potato, Solanum tuberosum L. Acta Physiologiae Plantarum, August 2014, Volume 36, Issue 8, pp 2059-2066.
Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.

application example

 

5 µg of stromal protein from (1) Chlamydomonas reinhardtii (left), (2) Arabidopsis thaliana   were separated on  SDS-PAGE. Primary antibodies have been used in 1: 3000.  

 

Western blot using anti-FeSOD antibodies
 

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