PRK ribulose-5-P-kinase | Phosphoribulokinase

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS07 257 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, H. vulgare, Z. mays, cyanobacteria, diatoms  | compartment marker of chloroplast stroma


33 st
Item No:
AS07 257

Info: Product suggestions Read reviews
product information

PRK ribulose-5-P-kinase| phosphoribulokinase is an enzyme that catalyzes the chemical reaction ATP + D-ribulose 5-phosphate to ADP + D-ribulose 1,5-bisphosphate


KLH-conjugated peptide derived from known PRK sequences including Arabidopsis thaliana P25697, At1g32060

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

collection of antibodies to various photosynthetic proteins

Additional information

Antibody can be used as a marker of chloroplast stroma.

application information
Recommended dilution

1: 1000 (WB)

Expected | apparent MW

44 | 39 kDa (A. thaliana)

Confirmed reactivity

Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Synechocystis PCC6803, Synechococcus PCC 7942, Thalassiosira pseudonana, Zea mays

Predicted reactivity

Glycine max, Hordeum vulgare, Malus domestica, Micromonas sp., Ostreocossus tauri, Oryza sativa, Physcomitrella patens, Populus trichocarpa, Spinacia oleracea, Solanum tuberosum,  Sorghum bicolor,  Synechocystis PCC 6803, Synechococcus elongatus, Zea mays, Vitis vinifera

Not reactive in


Additional information

antibody detects PRK using a load from 4-20 µg/well of a chloroplast fraction, incubation ON at 4°C.

Selected references to be added when available, antibody back in stock in February 2015

applicaton example

western blot using anti-PRK antibodies
WT-Col-0 Arabidopsis thaliana leaves were frozen in liquid nitrogen and soluble proteins were extracted in a buffer containing 50 mM HEPES, 5 mM NaCl and 10 mM MgCl2. 10 µg, 20 or 40 ug of eparated in SDS-PAGE (15% acrylamide with 6M urea) and blotted 1h to a PVDF membrane using Höefer semi-dry blotter. Blot was blocked with 4 % milk in TTBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight in +4°C. The antibody solution was decanted and the blot was washed 3 x 5 min with TTBS at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in 1% milk/TTBS for 2h at RT with agitation. The blot was washed 3x5 min in TTBS and 1x5 min in TBS at RT with agitation and developed for 5 min with ECL according to the manufacturer's instructions.

Courtesy of Lauri Nikkanen, University of Turku, Finland

||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at