PsbO1 | 33 kDa of the oxygen evolving complex (OEC) of PSII
AS14 2824 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana
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1: 1000 with standard ECL (WB)
|Expected | apparent MW||
33 | 30 kDa
|Predicted reactivity||Brasica napus, Capsella rubella, Triticum aestivum|
|Not reactive in||Oryza sativa|
This antibody is specific to PsbO1 and does not react with PsbO2 as confirmed on a deletion mutant.
|Selected references||to be added when available, antibody released in January 2015|
30 µg of thylakoid protein from Arabidopsis thaliana Columbia-0 extracted with 330 mM sorbitol, 50 mM Hepes-KOH (pH 7.8) were separated on 15% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 6h at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in 1% milk (TBS-T) for 2h at 4°C with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 60 seconds.
Courtesy of Dr. Peter Gollan, University of Turku, Finland
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