ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

PsbO1 | 33 kDa of the oxygen evolving complex (OEC) of PSII

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS14 2824  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
27 st
Delivery:
Shipping:
Item No:
AS14 2824

Info: Product suggestions Add review
product information
Background

The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include  33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II,  OEC 33 kDa subunit, 33 kDa thylakoid membrane protein,   manganese-stabilizing protein 2.

Immunogen

KLH-conjugated peptide chosen chosen from Arabidopsis thaliana PsbO1, UniProt: P23321,  TAIR:At5g66570 

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunoprecipitation (IP)
Related products

AS14 2825 | anti-PsbO2 | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS05 092 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibody

AS06 142-33 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibody

AS06 167 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibody

AS08 305 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibody

AS06 142-16 | anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 1000 with standard ECL (WB)

Expected | apparent MW

33 | 30 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Brasica napus, Capsella rubella, Triticum aestivum
Not reactive in Oryza sativa
Additional information

This antibody is specific to PsbO1 and does not react with PsbO2 as confirmed on a deletion mutant.

Selected references to be added when available, antibody released in January 2015

application example

western blot using anti-PsbO1 antibodies

30 µg of thylakoid protein from Arabidopsis thaliana Columbia-0 extracted with 330 mM sorbitol, 50 mM Hepes-KOH (pH 7.8) were separated on 15% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 6h at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in 1% milk (TBS-T) for 2h at 4°C with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 60 seconds.

Courtesy of Dr. Peter Gollan, University of Turku, Finland

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com