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PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII

345 €

AS08 305  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana, S. bicolor, Z. mays

PRODUCT INFORMATION IN PDF

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Item No:
AS08 305

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product information
Background

PSII reaction centre components are  generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.

Immunogen

Recombinant sorghum OE23 protein

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products

AS05 092 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII

AS06 142-33 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII

AS06 167 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII

AS06 142-16 | anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII

Additional information
application information
Recommended dilution

1: 5 000 with standard ECL (WB)

Expected | apparent MW

23 kDa

Confirmed reactivity Arabidopsis thaliana, Sorghum bicolor, Zea mays
Predicted reactivity

monocot including: Hordeum vulgare, Oryza sativa

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references

to be added when available


application example

 

 
 
5 µg of total protein from (1) Arabidopsis thaliana total leaf extract; (2) Zea mays total leaf  extract were separated in a 12% or 5-15% gradient gel following by transfer to nitrocellulose membrane. Membrane was blocked with TBST + 4% non-fat dried milk, 20 min following by three washes in TBST. Incubation time with primary and secondary antibodies was 1 hr primary, 30 min for secondary antibodies. Manufacturer of secondary antibodies: Bio-Rad
AS08 305 PsbP western.jpg


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