SHMT | Serine hydroxymethyltransferase

355 €

AS05 075  |  clonality: polyclonal  |  host: rabbit  |  reactivity:  A. thaliana, P. sativum, S. oleracea, V. faba  |  cellular[compartment marker] of mitochondrial matrix


3 st
Item No:
AS05 075

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product information

Serine hydroxymethyltransferase (SHMT) is part of the mitochondrial enzyme complex.  


Purified SHMT protein from Spinacia oleracea

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 200 µl
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 5 000 (WB)
Expected | apparent MW

53 kDa (Arabidopsis thaliana)

Confirmed reactivity Arabidopsis thaliana, Oryza sativa, Pisum sativum, Spinacia oleracea, Vicia faba
Predicted reactivity Chlamydomonas reinhardii, Glycine max, Hordeum vulgare, Medicago truncatula, Populus balsamifera, Ricinus communis, Solanum tuberosum, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

This antibody can be used on total leaf extracts and isolated mitochondria

Selected references Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.
Long et al. (2015). Contributions of photosynthetic and non-photosynthetic cell types to leaf respiration in Vicia faba L. and their responses to growth temperature. Plant Cell Environ. 2015 Apr 1. doi: 10.1111/pce.12544.
Wei et al. (2013). Folate polyglutamylation eliminates dependence of activity on enzyme concentration in mitochondrial serine hydroxymethyltransferases from Arabidopsis thaliana. Arch Biochem Biophys 1;536(1):87-96.
Camejo et al. (2012).Salinity-inducedchanges inS-nitrosylation ofpeamitochondrialproteins. J. Proteomics Dec.11.

application example



total leaf extract from Arabidopsis thaliana has been loaded per lane based on a leaf disc area. Primary antibody has been used in dilution 1: 5000 following detection using standard ECL. c


western blot detection using anti-SHMT antibody

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