Toc75 | Chloroplast outer envelope membrane translocon complex OEP75 protein

350 €

AS06 150 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, P. sativum, V. vinifera | cellular [compartment marker] of chloroplast outer envelope membrane


61 st
Item No:
AS06 150

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product information

Import of protein precurors into chloroplasts occurs via translocon complexes at the outer (Toc/TOC complex) and inner (Tic/TIC complex) envelope membrane. The major components of the Toc (TOC) complex in plants are Toc75, Toc34 and Toc159 (formerly Toc86). Toc75 (OEP75) forms the main import pore. It is an abundant protein in the outer envelope membrane of chloroplasts from expanding tissues but it is also found in plastids of roots, stems and flowers.


KLH-conjugated synthetic peptide conserved in available Toc75 (OPE75) sequences from monocots and dicots including Arabidopsis thaliana (Q9STE8, At3g46740). The Arabidopsis genome contains three Toc75-related sequences, termed atTOC75-I, atTOC75-III, and atTOC75-IV with atToc75-III being the most expressed one.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS08 292 | anti-Toc75 (Pisum sativum)

AS07 239 | anti-Toc159 (guinea pig antibody)

Plant protein extraction buffer

Secondary antibodies

Additional information

Toc75 can be used as a cellular [compartment marker] of chloroplast outer envelope membrane.

application information
Recommended dilution

1 : 1000 - 1: 5000 with standard ECL (WB)

Expected | apparent MW

89 kDa (with transit peptide) | 75 kDa (for Arabidopsis thaliana)

Confirmed reactivity

Arabidopsis thaliana, Nicotiana tabacum, Oryza sativa, Pisum sativum

Predicted reactivity

Brachypodium distachyon, Brassica napus, Brassica rapa subsp. pekinensis, Citrus clementina, Citrus sinensis, Coffea canephora, Eucalyptus grandis, Glycine max, Glycine soja, Hordeum vulgare var. distichum, Medicago truncatula,  Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolorTheobroma cacao, Triticum aestivum, Vitis vinifera, Populus sp., Prunus persicaZea mays,

magnoliophyta: Citrus sp., moss: Physcomitrella patens

Not reactive in

Chlamydomonas reinhardtii

Additional information
Selected references Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843.
Wang et al. (2014). Maintenance of chloroplast structure and function by overexpression of the OsMGD gene leads to enhanced salt tolerance in tobacco. Plant Physiol. 2014 May 19. pii: pp.114.238899.
Brillouet et al. (2013).The tannosome is an organelle forming condensed tannins in the chlorophyllous organs of Tracheophyta. Ann Bot. Sep. 11. (Vitis vinifera, immunofluorescence)

application example

10 µg protein from (1) Arabidopsis thaliana leaf extracted with PEB (AS08 300), (2) chloplast envelope membranes, and (3) thylakoids were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Toc75 (AS06 150, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (Invitrogen) using a Fuji LAS-3000 CCD (300s, standard sensitivity).

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