Toc75 | Chloroplast outer envelope membrane translocon complex OEP75 protein
AS06 150 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, P. sativum, V. vinifera | cellular [compartment marker] of chloroplast outer envelope membrane
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1 : 1000 - 1: 5000 with standard ECL (WB)
|Expected | apparent MW||
89 kDa (with transit peptide) | 75 kDa (for Arabidopsis thaliana)
Arabidopsis thaliana, Nicotiana tabacum, Oryza sativa, Pisum sativum
Brachypodium distachyon, Brassica napus, Brassica rapa subsp. pekinensis, Citrus clementina, Citrus sinensis, Coffea canephora, Eucalyptus grandis, Glycine max, Glycine soja, Hordeum vulgare var. distichum, Medicago truncatula, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera, Populus sp., Prunus persica, Zea mays,
|Not reactive in||
|Selected references||Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843.
Wang et al. (2014). Maintenance of chloroplast structure and function by overexpression of the OsMGD gene leads to enhanced salt tolerance in tobacco. Plant Physiol. 2014 May 19. pii: pp.114.238899.
Brillouet et al. (2013).The tannosome is an organelle forming condensed tannins in the chlorophyllous organs of Tracheophyta. Ann Bot. Sep. 11. (Vitis vinifera, immunofluorescence)
10 µg protein from (1) Arabidopsis thaliana leaf extracted with PEB (AS08 300), (2) chloplast envelope membranes, and (3) thylakoids were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Toc75 (AS06 150, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (Invitrogen) using a Fuji LAS-3000 CCD (300s, standard sensitivity).
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