Protocols and technical information

Protocols	Technical information
ELISA Short protocol & trouble shooting	IgG
Immunoprecipitation Trouble shooting	IgM
Immunoprecipitation using IgY	IgY
Immunohistochemistry using IgY	IgY purification
Peptide neutralisation/competition assay	Protein purification using antibodies
Removal of cross-reacting KLH antibodies	Elution of antibodies from affinity columns
Western blot trouble shooting	Problems with dialysis
Western Blot using IgY	Affinity purification of antibodies - small amount of protein available
Yolk delipidation	Antibody storage
	Antibody production guide

Agrisera video tutorial: How to make a good western blot? (qualitative and quantitative)

Useful basic reference to work with antibodies:

Using Antibodies: A Laboratory Manual, E.Harlow and D.Lane, 1999,
ISBN: 0879695447; Publisher: Cold Spring Harbor Laboratory Press.

Protocols