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Protocols and technical information

Protocols

                       

Technical information

ELISA Short protocol and trouble shooting                  

Immunoprecipitation Trouble shooting

Peptide neutralisation/competition assay Confirmation that antibody is recognizing target protein 

Removal of cross-reacting KLH antibodies

Western blot trouble shooting Tips, Agrisera western blot protocol, antibody storage information

Immunohistochemistry using IgY

Immunoprecipitation using IgY

Western Blot using IgY

Yolk delipidation  

     

IgG

IgM

IgY

IgY purification

Protein purification using antibodies

Elution of antibodies from affinity columns

Problems with dialysis

Affinity purification of antibodies - small amount of protein available

Agrisera video tutorial: How to make a good western blot? (qualitative and quantitative)

Useful basic reference to work with antibodies:

Using Antibodies: A Laboratory Manual, E.Harlow and D.Lane, 1999,
ISBN: 0879695447; Publisher: Cold Spring Harbor Laboratory Press.

Useful link: Antibody resource page
Agrisera AB Box 57, SE-911 21 Vännäs, SWEDEN | Phone: +46(0)935 33000 | Fax: +46(0)935 33044 | VAT nr: SE556263276901 | OLAW/NIH nr: A5783-01 | info@agrisera.com